Interaction of Campylobacter spp. and Human Probiotics in Chicken Intestinal Mucus

Authors

  • M. Ganan,

    1.  Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Madrid, Spain
    2.  Departamento de Microbiología, Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain
    3.  Functional Foods Forum, University of Turku, Turku, Finland
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  • A. J. Martinez-Rodriguez,

    1.  Departamento de Microbiología, Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain
    2.  Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, Madrid, Spain
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  • A.V. Carrascosa,

    1.  Departamento de Microbiología, Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain
    2.  Instituto de Investigación en Ciencias de la Alimentación (CIAL), CSIC-UAM, Madrid, Spain
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  • S. Vesterlund,

    1.  Functional Foods Forum, University of Turku, Turku, Finland
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  • S. Salminen,

    1.  Functional Foods Forum, University of Turku, Turku, Finland
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  • R. Satokari

    1.  Functional Foods Forum, University of Turku, Turku, Finland
    2.  Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland
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M. Ganan. Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Av. Puerta de Hierro, s/n. 28040. Madrid, Spain. Tel.: +34 913943749; Fax: +34 91 3943743; E-mail: mganan@vet.ucm.es

Summary

Campylobacter is the most common cause of bacterial food-borne diarrhoeal disease throughout the world. The principal risk of human contamination is handling and consumption of contaminated poultry meat. To colonize poultry, Campylobacter adheres to and persists in the mucus layer that covers the intestinal epithelium. Inhibiting adhesion to the mucus could prevent colonization of the intestine. The aim of this study was to investigate in vitro the protective effect of defined commercial human probiotic strains on the adhesion of Campylobacter spp. to chicken intestinal mucus, in a search for alternatives to antibiotics to control this food-borne pathogen. The probiotic strains Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. shermanii JS and a starter culture strain Lactococcus lactis ssp. lactis adhered well to chicken intestinal mucus and were able to reduce the binding of Campylobacter spp. when the mucus was colonized with the probiotic strain before contacting the pathogen. Human-intended probiotics could be useful as prophylactics in poultry feeding for controlling Campylobacter spp. colonization.

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