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Detection of Brucella spp. DNA in the semen of seronegative bulls by polymerase chain reaction

Authors

  • D. G. Junqueira Junior,

    1. Faculty of Veterinary Medicine, Collaborating Centre of Agricultural Protection in Central Brazil, Federal University of Uberlândia, Uberlândia, MG, Brazil
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  • G. M. S. Rosinha,

    1. Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, National Center for Research in Beef Cattle, Campo Grande, Mato Grosso do Sul, Brazil
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  • C. E. G. Carvalho,

    1. Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, National Center for Research in Beef Cattle, Campo Grande, Mato Grosso do Sul, Brazil
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  • C. E. Oliveira,

    1. Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, National Center for Research in Beef Cattle, Campo Grande, Mato Grosso do Sul, Brazil
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  • C. C. Sanches,

    1. Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, National Center for Research in Beef Cattle, Campo Grande, Mato Grosso do Sul, Brazil
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  • A. M. C. Lima-Ribeiro

    1. Faculty of Veterinary Medicine, Collaborating Centre of Agricultural Protection in Central Brazil, Federal University of Uberlândia, Uberlândia, MG, Brazil
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  • Danilo Guedes Junqueira Júnior, St. Ceará, s/n, 2D-33, Uberlândia, MG, Brazil, 38400-902. Federal University of Uberlandia. Place where the work was carried out: Empresa Brasileira de Pesquisa Agropecuária.

D. G. Junqueira Junior. Faculty of Veterinary Medicine, Collaborating Centre of Agricultural Protection in Central Brazil, Federal University of Uberlândia, St. Ceará, s/n, 2D-33, 38400-902 Uberlândia, MG, Brazil. Tel.: +55 34 3218 2227; Fax: +55 34 3218 2228; E-mail: dan_hp2002@yahoo.com.br

Summary

Semen samples from 88 reproductively mature bulls were screened to detect the presence of Brucella spp. by polymerase chain reaction. Twenty-seven samples were found to be positive, underscoring the importance of researching brucellosis in males and the need for greater care in the selection of sperm-donating bulls for semen centres.

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