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Figure S1 Expression patterns of lineage marker genes during hESC differentiation. (a) Transcriptional expression of lineage marker genes in hESC-derived DE cells. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was used as a control. Assayed genes: SOX17 (SRY-box 17), GATA4 (GATA-binding protein 4), FOXA2 (forkhead box A2), CXCR4 (chemokine (C-X-C motif) receptor 4), OCT4 (POU class 5 homeobox 1), T (T, brachyury homolog), MEOX1 (mesenchyme homeobox 1), PAX6 (paired box 6), ZIC1 (Zic family member 1), SOX1 (SRY-box 1), NESTIN , SOX7 (SRY-box 7), and AFP (alpha-fetoprotein). (b) Transcriptional expression of hepatocyte marker genes in hESC-derived hepatocytes and HepG2 cells: ALB (albumin), AFP (alpha-fetoprotein), AAT (alpha-1 antitrypsin), CYP2B6 (cytochrome P450, family 2, subfamily B, polypeptide 6), CYP3A4 (cytochrome P450, family 3, subfamily A, polypeptide 4), CYP7A1 (cytochrome P450, family 7, subfamily a, polypeptide 1), G6P (glucose-6-phosphatase), HNF4A (hepatocyte nuclear factor 4, alpha).

Figure S2 Correlation of miRNAs between duplicates.

Figure S3 Relative expression patterns of representative miRNAs between microarray and quantitative RT-PCR.

Figure S4 Expression patterns of let-7a and let-7f between hESC lines. (a) A heatmap of let-7a and let-7f from the miRNA array data. (b) Relative expression levels of let-7a and let-7f by quantitative RT-PCR between two hESC lines, CHA4 and H9.

Figure S5 Enhanced expression of putative target genes of miR-520b and miR-525-5p during differentiation of hESCs into hepatocytes. Relative expression levels compared to hESCs are shown.

Figure S6 Expression levels of PTEN. (a) mRNA expression levels of PTEN in hESC, DE cells, and hepatocytes. (b) Western blot result of PTEN in hESC, DE cells, and hepatocytes.

Table S1 Primers used in this study

Table S2 Downregulated microRNAs during transition of hESCs to DE cells

Table S3 Upregulated microRNAs during transition of hESCs to DE cells

Table S4 Downregulated microRNAs during differentiation of DE cells to hepatocytes

Table S5 Upregulated microRNAs during differentiation of DE cells to hepatocytes

FilenameFormatSizeDescription
HEPR_752_sm_supf1.tif216KSupporting info item
HEPR_752_sm_supf2.tif291KSupporting info item
HEPR_752_sm_supf3.tif562KSupporting info item
HEPR_752_sm_supf4.tif209KSupporting info item
HEPR_752_sm_supf5.tif99KSupporting info item
HEPR_752_sm_supf6.tif40KSupporting info item
HEPR_752_sm_suptbls1-5.doc850KSupporting info item

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