Morphologic, immunohistochemical, and molecular characterization of hepatosplenic T-cell lymphoma in a dog


Corresponding author: Elizabeth A. Cienava, DVM, Department of Veterinary Pathobiology, Texas A&M University, Veterinary Medical Teaching Hospital, Clinical Pathology, Bldg 1085, Room 2020, College Station, TX 77843–9988 (


Abstract: A 13–year-old neutered male Jack Russell Terrier (Parson Russell Terrier) was presented to the Texas Veterinary Medical Center with a history of lethargy, depression, vomiting, and fever. The dog had mildly regenerative anemia, severe thrombocytopenia and low antithrombin activity. Marked splenomegaly was found on physical examination and imaging studies, and malignant round cell neoplasia and marked extramedullary hematopoiesis were diagnosed on aspirates of the spleen. The dog underwent exploratory laporatomy and splenectomy. Because of a rapid decline in clinical condition post-surgery the dog was euthanized. Splenic and hepatic biopsies were submitted for histopathologic evaluation. A neoplastic population of round cells was found throughout the splenic parenchyma and within hepatic sinusoids. The neoplastic cells stained strongly positive for CDS (T-cell marker) and were negative for CD79a (B-cell marker) and lysozyme (histiocytic marker). A diagnosis of T-cell lymphoma was confirmed by assessment of T-cell clonality using canine-specific polymerase chain reaction-based techniques. Although expression of the γδ T-cell receptor was not evaluated, this case shares many similarities with a rare syndrome in humans known as hepatosplenic γδ T-cell lymphoma.