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Clinical evaluation of the CA530-VET hematology analyzer for use in veterinary practice

Authors

  • Sabine Roleff,

    1. From the Clinic for Small Animals (Roleff, Kohn), the Department of Biometrics (Arndt), and the Clinic for Equine Medicine (Grabner) of the Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany; the Boule Medical AB, Stockholm, Sweden (Bottema); and Menarini Diagnostics, Neuss, Germany (Junker). Dr Junker now is with Instrumentation Laboratory, Kirchheim, Germany. Preliminary results of this study were presented at the 12th Annual Conference for Internal Medicine and Clinical Laboratory Diagnostics, German Veterinary Medical Society, February 1–2, 2003, Munich, Germany. Corresponding author: Barbara Kohn, DMV (kohn.barbara@vetmed.fu-berlin.de)
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  • Gisela Arndt,

    1. From the Clinic for Small Animals (Roleff, Kohn), the Department of Biometrics (Arndt), and the Clinic for Equine Medicine (Grabner) of the Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany; the Boule Medical AB, Stockholm, Sweden (Bottema); and Menarini Diagnostics, Neuss, Germany (Junker). Dr Junker now is with Instrumentation Laboratory, Kirchheim, Germany. Preliminary results of this study were presented at the 12th Annual Conference for Internal Medicine and Clinical Laboratory Diagnostics, German Veterinary Medical Society, February 1–2, 2003, Munich, Germany. Corresponding author: Barbara Kohn, DMV (kohn.barbara@vetmed.fu-berlin.de)
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  • Bram Bottema,

    1. From the Clinic for Small Animals (Roleff, Kohn), the Department of Biometrics (Arndt), and the Clinic for Equine Medicine (Grabner) of the Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany; the Boule Medical AB, Stockholm, Sweden (Bottema); and Menarini Diagnostics, Neuss, Germany (Junker). Dr Junker now is with Instrumentation Laboratory, Kirchheim, Germany. Preliminary results of this study were presented at the 12th Annual Conference for Internal Medicine and Clinical Laboratory Diagnostics, German Veterinary Medical Society, February 1–2, 2003, Munich, Germany. Corresponding author: Barbara Kohn, DMV (kohn.barbara@vetmed.fu-berlin.de)
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  • Lothar Junker,

    1. From the Clinic for Small Animals (Roleff, Kohn), the Department of Biometrics (Arndt), and the Clinic for Equine Medicine (Grabner) of the Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany; the Boule Medical AB, Stockholm, Sweden (Bottema); and Menarini Diagnostics, Neuss, Germany (Junker). Dr Junker now is with Instrumentation Laboratory, Kirchheim, Germany. Preliminary results of this study were presented at the 12th Annual Conference for Internal Medicine and Clinical Laboratory Diagnostics, German Veterinary Medical Society, February 1–2, 2003, Munich, Germany. Corresponding author: Barbara Kohn, DMV (kohn.barbara@vetmed.fu-berlin.de)
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  • Arthur Grabner,

    1. From the Clinic for Small Animals (Roleff, Kohn), the Department of Biometrics (Arndt), and the Clinic for Equine Medicine (Grabner) of the Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany; the Boule Medical AB, Stockholm, Sweden (Bottema); and Menarini Diagnostics, Neuss, Germany (Junker). Dr Junker now is with Instrumentation Laboratory, Kirchheim, Germany. Preliminary results of this study were presented at the 12th Annual Conference for Internal Medicine and Clinical Laboratory Diagnostics, German Veterinary Medical Society, February 1–2, 2003, Munich, Germany. Corresponding author: Barbara Kohn, DMV (kohn.barbara@vetmed.fu-berlin.de)
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  • Barbara Kohn

    1. From the Clinic for Small Animals (Roleff, Kohn), the Department of Biometrics (Arndt), and the Clinic for Equine Medicine (Grabner) of the Free University of Berlin, Oertzenweg 19b, 14163 Berlin, Germany; the Boule Medical AB, Stockholm, Sweden (Bottema); and Menarini Diagnostics, Neuss, Germany (Junker). Dr Junker now is with Instrumentation Laboratory, Kirchheim, Germany. Preliminary results of this study were presented at the 12th Annual Conference for Internal Medicine and Clinical Laboratory Diagnostics, German Veterinary Medical Society, February 1–2, 2003, Munich, Germany. Corresponding author: Barbara Kohn, DMV (kohn.barbara@vetmed.fu-berlin.de)
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Abstract

Background: The CA530-VET is a completely automated impedance cell hematology analyzer, which yields a 16-parameter blood count including a 3-part leukocyte differential. Objectives: The aim of this study was to examine the operational potential of the CA530-VET and its value for use in veterinary practice. Methods: The analyzer was tested for blood carry-over, precision, and accuracy. Comparison methods included the CELL-DYN 3500, microhematocrit centrifugation, manual platelet (PLT) counting for feline and equine species, and a 100-cell manual WBC differential. Blood samples for comparison of the methods were obtained from 242 dogs, 166 cats, and 144 horses. Results: The carry-over ratio (K) was 0.28% for RBC, 0.59% for PLT, 0.32% for WBC, and 0.18% for hemoglobin (HGB) concentration. Coefficients of variation (CVs) for within-batch precision and duplicate measurement of blood samples were clearly within the required limits, except for duplicate platelet counts in cats (8.7%) and horses (9.5%). The WBC count was in excellent agreement for dogs and horses and RBC count was in excellent agreement for horses. The accuracy of feline WBC counts was not acceptable, with the exception of values at the high end of the range. RBC counts in dogs and cats, and HGB concentration and MCV in all 3 species were sufficiently accurate. The CA530-VET HCT results were in excellent agreement with microhematocrit results in horses but exceeded the maximum allowed inaccuracy for cats and dogs. In all species, PLT counts established mechanically and manually were not in adequate agreement. Large differences were found between the CA530-VET and the manual differential percentage for lymphocytes and “mid-sized cells” (monocytes and basophilic granulocytes). Conclusions: The CA530-VET can be considered useful for routine canine, feline, and equine blood cell analyses. It should not be considered accurate, however, for PLT counts, feline total WBC counts in the subnormal and normal range, and leukocyte differentials, except for granulocytes.

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