Changes in gas composition and acid-base values of venous blood samples stored under different conditions in 4 domestic species

Authors

  • Giuseppe Piccione,

    1. From the Department of Morphology, Biochemistry, Physiology and Animal Production (Piccione, Grasso) and the Department of Veterinary Public Health (Giudice), Faculty of Veterinary Medicine, University of Messina, Messina, Italy; and the Department of Biology and Evolution, University of Ferrara, Ferrara, Italy (Bertolucci). The first 2 authors contributed equally to this work. Corresponding author: Giuseppe Piccione (giuseppe.piccione@unime.it)
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  • Cristiano Bertolucci,

    1. From the Department of Morphology, Biochemistry, Physiology and Animal Production (Piccione, Grasso) and the Department of Veterinary Public Health (Giudice), Faculty of Veterinary Medicine, University of Messina, Messina, Italy; and the Department of Biology and Evolution, University of Ferrara, Ferrara, Italy (Bertolucci). The first 2 authors contributed equally to this work. Corresponding author: Giuseppe Piccione (giuseppe.piccione@unime.it)
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  • Fortunata Grasso,

    1. From the Department of Morphology, Biochemistry, Physiology and Animal Production (Piccione, Grasso) and the Department of Veterinary Public Health (Giudice), Faculty of Veterinary Medicine, University of Messina, Messina, Italy; and the Department of Biology and Evolution, University of Ferrara, Ferrara, Italy (Bertolucci). The first 2 authors contributed equally to this work. Corresponding author: Giuseppe Piccione (giuseppe.piccione@unime.it)
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  • Elisabetta Giudice

    1. From the Department of Morphology, Biochemistry, Physiology and Animal Production (Piccione, Grasso) and the Department of Veterinary Public Health (Giudice), Faculty of Veterinary Medicine, University of Messina, Messina, Italy; and the Department of Biology and Evolution, University of Ferrara, Ferrara, Italy (Bertolucci). The first 2 authors contributed equally to this work. Corresponding author: Giuseppe Piccione (giuseppe.piccione@unime.it)
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Abstract

Background: The effect of storage temperature and time on blood gas and acid-base values has been investigated intensively in cattle and dogs; however, data are lacking in other species. Objective: The aim of our study was to evaluate changes in gas composition and acid-base values in venous blood stored at different temperatures and for different times in 4 domestic species in Italy. Methods: Blood samples from Comisana sheep (n=10), Maltese goats (n=10), Ragusana donkeys (n=10), and Thoroughbred horses (n=10) were analyzed after storage at 23°C (room temperature) for 15 minutes (group I), 23°C for 1 hour (group II), 37°C for 8 hours (group III), and 4°C for 24 hours (group IV). Results were analyzed using a 1-way repeated measures ANOVA. Results: In all species no statistically significant differences in pH values were present in samples stored at 4°C for 24 hours. This also was true for Pco2 in all species except the horse. Except for HCO3 concentration in the horse, significant changes in Po2, HCO3 concentration, base excess, and the standard bicarbonate concentration were observed for all species in samples stored at 4°C. In samples stored for only 1 hour at room temperature, significant changes in most analytes were detected. Conclusions: The results of this study underline the need for rapid assessment of acid-base samples, because any delay, even for 1 hour, may affect the results.

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