vcp455-sup-0001-FigureS1.pdfapplication/PDF1004KFigure S1. Platelet graphics from a Cavalier King Charles Spaniel (CKCS) dog (right) with macrothrombocytopenia and a German Shepherd dog (left) with normal appearing platelets. The CKCS dog has larger platelets as indicated by the green ovals. Note in the PLT SCATTER dot plots, most of the blue dots (platelets) in the German Shepherd are located low in the dot plot, whereas in the CKCS the dots are located higher and to the right in the cytograms, including along the upper and right edges of the plot. Platelets between 30 and 60 fL are located along the upper border of the dot plot. The PLT VOL histogram at the bottom shows a normal pattern for the German Shepherd with most platelets in a peak on the left, whereas for the CKCS dog the main peak is shifted to the right (larger volume) and individual bars to the far right represent fewer very large platelets. The PLT VOL PC dot plot shows an enlarged view of the region between platelets (blue dots) and erythrocytes (red dots) with platelets from the CKCS being closer to erythrocytes, but still with minimal overlap between platelets and erythrocytes, suggesting correct identification of platelets and erythrocytes in the sample.
vcp455-sup-0002-FigureS2.pdfapplication/PDF1004KFigures S2. RBC scatter dot plots and PLT VOL PC plots from a Cavalier King Charles Spaniel (CKCS) dog (A) and a normal dog (B). The RBC scatter dot plot reflects increasing volume along the y-axis and optical density along the x-axis. The CKSC dog had very large platelets (within the blue oval) extending up into the area where leukocytes are found. Leukocytes in the RBC scatter dot plot from the normal dog are indicated by the red oval; platelets are in the small blue oval. Even though platelets from the CKCS dog were larger than erythrocytes, their lower optical density (black dots are more to the left along the x-axis) separates them from erythrocytes (red dots within the Mie grid). However, the largest platelets were not clearly separated from leukocytes. The PLT VOL PC expands the area between platelets and erythrocytes. The large platelets are within the green oval.
vcp455-sup-0003-FigureS3.pdfapplication/PDF1004KFigure S3. Passing-Bablok (A) and difference (B) plots comparing converted Advia plateletcrits (PCT × 1000) and QBC calculated platelet counts in 43 Cavalier King Charles Spaniel dogs. Gray lines are the lines of identity. (A) Proportional bias of the converted Advia PCT is similar to that shown in Figure 1B. (B) The negative bias for converted Advia PCT was 26 × 109/L. The blue solid line indicates the negative bias, and the green dashed lines indicate the 95% limits of agreement. Numbers on the x- and y-axes are platelets × 109/L.
vcp455-sup-0004-FigureS4.pdfapplication/PDF1004KFigure S4. Advia platelet counts (×109/L) and MPVs (fL × 10) for 43 Cavalier King Charles Spaniel dogs. The MPVs were higher in dogs with macrothrombocytopenia when platelet counts were < 146 × 109/L. Advia MPV followed the expected biologic pattern in this disorder, supporting at least partial accuracy of the Advia MPV measurement. The MPV values > 145 on the y-axis (10× the upper limit of the reference value, 14.5 fL) were increased.
vcp455-sup-0005-FigureS5.pdfapplication/PDF1004KFigure S5. Stability of Advia plateletcrit (PCT) (A), MPV (B), and platelet count (C) measured in EDTA blood collected from 7 dogs (7 colored lines) and analyzed within 2 hours of collection (day 0) or after storage for 1 and 2 days at room temperature (17–22°C). See text for details. Stability was not optimal, but was adequate for analysis of samples sent by mail. One dog (orange line) had marked thrombocytosis and a high PCT.

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