Part of this study was presented at the 16th annual ACVIM Forum in San Diego, CA, May 22–25, 1998.
Plasma Exogenous Creatinine Clearance Test in Dogs: Comparison with Other Methods and Proposed Limited Sampling Strategy
Article first published online: 28 JUN 2008
Journal of Veterinary Internal Medicine
Volume 16, Issue 1, pages 22–33, January 2002
How to Cite
Watson, A.D.J., Lefebvre, H. P., Concordet, D., Laroute, V., Ferré, J.-P., Braun, J.-P., Conchou, F. and Toutain, P.-L. (2002), Plasma Exogenous Creatinine Clearance Test in Dogs: Comparison with Other Methods and Proposed Limited Sampling Strategy. Journal of Veterinary Internal Medicine, 16: 22–33. doi: 10.1111/j.1939-1676.2002.tb01603.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Revised May 3, 2001; Accepted July 26, 2001
- Glomerular fitration rate;
Plasma clearance of creatinine was evaluated for assessment of glomerular nitration rate (GFR) in dogs. In 6 healthy dogs (Experiment 1), we determined 24-hour urine clearance of endogenous creatinine, plasma, and urine clearances of exogenous creatinine administered at 40, 80, and 160 mg/kg in a crossover design (linearity study), plasma iothalamate clearance, and plasma and urine clearances of 14C-inulin. In Experiment 2, plasma creatinine and iothalamate clearances were compared, and a linearity study was performed as for Experiment 1 in 6 dogs with surgically induced renal impairment. Experiment 3 compared plasma creatinine clearance with plasma iothalamate clearance before and 3 weeks after induction of moderate renal impairment in 6 dogs. Plasma creatinine clearances were calculated by both noncompartmental and compartmental analyses. In Experiment 1, plasma inulin clearance was higher (P < .001) than other clearance values. Plasma creatinine clearances at the 3 dose rates did not differ from urine inulin clearance and each other. In Experiment 2, plasma creatinine clearances were about 14% lower than plasma iothalamate clearance (P < .05). In Experiment 3, decreases in GFR assessed by plasma clearances of iothalamate and creatinine were similar. Renal failure decreased the daily endogenous input rate of creatinine by 25%. Limiting sampling strategies for optimizing GFR calculation were proposed, allowing an error lower than 6.5% with 4 blood samples. These results suggest that determination of plasma creatinine clearance by a noncompartmental approach offers a reliable, inexpensive, rapid, and convenient means of estimating GFR in routine practice.