Cytokine mRNA Quantification in Duodenal Mucosa from Dogs with Chronic Enteropathies by Real-Time Reverse Transcriptase Polymerase Chain Reaction
Version of Record online: 5 FEB 2008
© 2005 American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 19, Issue 5, pages 644–653, September 2005
How to Cite
Peters, I. R., Helps, C. R., Calvert, E. L., Hall, E. J. and Day, M. J. (2005), Cytokine mRNA Quantification in Duodenal Mucosa from Dogs with Chronic Enteropathies by Real-Time Reverse Transcriptase Polymerase Chain Reaction. Journal of Veterinary Internal Medicine, 19: 644–653. doi: 10.1111/j.1939-1676.2005.tb02742.x
- Issue online: 5 FEB 2008
- Version of Record online: 5 FEB 2008
- Submitted June 28, 2004; Revised October 23, 2004; Accepted April 14, 2005.
Vol. 30, Issue 1, 469, Version of Record online: 17 DEC 2015
- Chronic Diarrhea;
- Inflammatory bowel disease (IBD);
The pathogenesis of inflammatory bowel disease (IBD) and antibiotic-responsive diarrhea (ARD) in dogs likely involves an interaction between the intestinal immune system and luminal bacterial or food antigens. German Shepherd Dogs (GSD) are particularly predisposed to both IBD and ARD. CD4+ T cells are important for the regulation of immune responses in the mucosa, and they exert their effects through the secretion of cytokines. The present study examined the role of cytokines in the pathogenesis of canine chronic enteropathies by quantification of mRNA encoding interleukin-2 (IL-2), IL-4, IL-5, IL-6, IL-10, IL-12, IL-18, interferon -y, tumor necrosis factor–α, transforming growth factor–β, and glyceraldehyde-3-phosphate dehydrogenase by real-time reverse transcriptase polymerase chain reaction in duodenal mucosal biopsies obtained from 39 dogs with chronic diarrhea and 18 control dogs. Contemporaneously collected biopsies were assessed for histologic changes with a 4-point grading system. No significant difference in the expression of cytokine mRNA (P > .01) was detected between dogs with and those without chronic diarrhea. Similarly, no significant differences in cytokine mRNA expression were observed between GSD and other breeds with chronic diarrhea, or between histologically normal duodenal mucosa and that with evidence of inflammatory change. Failure to detect a difference in mRNA expression does not rule out the possibility of a defect downstream at the level of translation or protein function. No conclusion can be drawn from these data as to the predominant CD4+ cell type in the pathogenesis of these canine chronic enteropathies.