Veterinarians involved in Greyhound rescue have anecdotally observed that 10–15% of Greyhounds bleed profusely after simple surgical procedures. In most patients, platelet counts and hemostasis profiles are normal; therefore, it is possible that these dogs have platelet dysfunction. The PFA-100a is a novel point-of-care platelet function analyzer that has recently been evaluated as a rapid method to assess platelet function in dogs. The objectives of this study were to characterize platelet function in a group of healthy Greyhounds by means of the PFA-100. Blood samples were collected from the jugular vein from 30 healthy Greyhounds. CBC, biochemical profile, PFA-100 assay with collagen/epinephrine (COL-EPI) and collagen/ adenosindiphosphate (COL-ADP), plasma von Willebrand factor antigen concentration (vWF: Ag), and vWF collagen-binding assay (vWF:CBA) were performed. PFA-100 closure times (CTs) with COL/ADP ranged from 63 to 92 seconds (mean ± SD, 74.7 ± 7.9 seconds) and with COL/EPI from 87 to 238 seconds (138 ± 41 seconds); vWF:Ag ranged from 22 to 120% (87.52 ± 25.5%) and vWF:CBA ranged from 36 to 102% (77.4 ± 17.3%); and platelet counts ranged from 147 to 265 ± 109/L (194.6 ± 31.64 ± 109/L). Greyhound CTs were significantly shorter than CTs in a mixed population of 50 healthy non-Greyhound dogs, in which the COL/ADP CTs ranged from 61 to 172 seconds (mean ± SD, 87 ± 21.6 seconds), and the COL/ EPI CTs ranged from 81 to 300 seconds (mean ± SD, 183 ± 67.6 seconds; P= 0.005 for COL/ADP CT; P= 0.001 for COL/ EPI CT). Also, platelet counts were significantly lower (P= 0.001) and packed cell volume was significantly higher (P= 0.001) in the Greyhound than in the non-Greyhound group. The PFA-100 is a reproducible method that can be used in the clinical setting to assess platelet function in Greyhounds; however, normal CTs in healthy Greyhounds are shorter than in other breeds. The results obtained in this study will be used to screen for abnormal platelet function in Greyhounds with postoperative bleeding.