• Open Access

Poor Reproducibility of Template Bleeding Time in Horses

Authors

  • D. Segura,

    1. Servei de Medicina Interna Equina, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain
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  • L. Monreal

    1. Servei de Medicina Interna Equina, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona, Spain
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Corresponding author: Luis Monreal, Servei de Medicina Interna Equina, Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain; e-mail: lluis.monreal@uab.es

Abstract

Background: Template bleeding time (TBT) is considered to be a useful test for detecting platelet function disorders and the effect of platelet-activating drugs, but studies in human medicine have concluded that the test has poor reproducibility and sensitivity.

Hypothesis: TBT has poor reproducibility in horses and has insufficient sensitivity to detect the effect of etamsylate on platelet function.

Animals: Twenty healthy horses.

Methods: TBT was determined and repeated 2 hours and 30 days later. TBT was also performed 2 hours after IV administration of etamsylate.

Results: Although no statistical differences were seen between the TBT values obtained at different times, the coefficients of variation for TBT replicates ranged from 26.8% to 45.5%. The reference range for TBT was 138.4–860.4 seconds. No statistically significant shortening of the mean TBT value was observed after etamsylate administration.

Conclusion and Clinical Importance: TBT has poor reproducibility, and the reference range is too wide to make TBT useful in a clinical setting. Other tests with higher reproducibility should be considered when assessing platelet function disorders in horses.

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