• Open Access

A Newly Recognized Blood Group in Domestic Shorthair Cats: The Mik Red Cell Antigen

Authors

  • Nicole M. Weinstein,

    1. Sections of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
    2. Penn Animal Blood Bank, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
    3. Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
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  • Marie-Claude Blais,

    1. Sections of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
    2. Penn Animal Blood Bank, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
    3. Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
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  • Kimberly Harris,

    1. Sections of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
    2. Columbus Central Veterinary Hospital, Columbus, NJ.
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  • Donna A. Oakley,

    1. Penn Animal Blood Bank, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
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  • Lillian R. Aronson,

    1. Surgery, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
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  • Urs Giger

    1. Sections of Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
    2. Penn Animal Blood Bank, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
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    • 2

      Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104–6010; e-mail: giger@vet.upenn.edu.


Abstract

Background:Naturally occurring alloantibodies produced against A and B red cell antigens in cats can cause acute hemolytic transfusion reactions. Blood incompatibilities, unrelated to the AB blood group system, have also been suspected after blood transfusions through routine crossmatch testing or as a result of hemolytic transfusion reactions.

Hypothesis:Incompatible crossmatch results among AB compatible cats signify the presence of a naturally occurring alloantibody against a newly identified blood antigen in a group of previously never transfused blood donor cats. The associated alloantibody is clinically important based upon a hemolytic transfusion reaction after inadvertent transfusion of red cells expressing this red cell antigen in a feline renal transplant recipient that lacks this red cell antigen.

Methods: Blood donor and nonblood donor cats were evaluated for the presence of auto-and alloantibodies using direct antiglobulin and crossmatch tests, respectively, and were blood typed for AB blood group status. Both standard tube and novel gel column techniques were used.

Results: Plasma from 3 of 65 cats and 1 feline renal transplant recipient caused incompatible crossmatch test results with AB compatible erythrocytes indicating these cats formed an alloantibody against a red cell antigen they lack, termed Mik. The 3 donors and the renal transplant recipient were crossmatch-compatible with one another. Tube and gel column crossmatch test results were similar.

Conclusions and Clinical Importance: The absence of this novel Mik red cell antigen can be associated with naturally occurring anti-Mik alloantibodies and can elicit an acute hemolytic transfusion reaction after an AB-matched blood transfusion.

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