Effect of a Short-Term Infusion with Soybean Oil-Based Lipid Emulsion on Phagocytic Responses of Canine Peripheral Blood Polymorphonuclear Neutrophilic Leukocytes
Article first published online: 4 AUG 2008
Copyright © 2008 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 22, Issue 5, pages 1166–1173, September–October 2008
How to Cite
Kang, J.-H. and Yang, M.-P. (2008), Effect of a Short-Term Infusion with Soybean Oil-Based Lipid Emulsion on Phagocytic Responses of Canine Peripheral Blood Polymorphonuclear Neutrophilic Leukocytes. Journal of Veterinary Internal Medicine, 22: 1166–1173. doi: 10.1111/j.1939-1676.2008.0159.x
- Issue published online: 25 AUG 2008
- Article first published online: 4 AUG 2008
- Submitted February 18, 2008; Revised April 28, 2008; Accepted June 13, 2008.
- Flow cytometry;
- Neutrophil function;
- Parenteral nutrition
Background: The use of soybean oil-based lipid emulsion (SO-based LE) in parenteral nutrition has been reported to impair neutrophil functions in humans and rodents. As yet, little is understood about the effects of SO-based LE on canine immune responses.
Hypothesis: A short-term infusion with SO-based LE affects the phagocytic responses of canine peripheral blood polymorphonuclear neutrophilic leukocytes (PMNs).
Animals: Twenty-four healthy Beagle dogs.
Methods: Experimental study. Dogs were randomly assigned into groups of six and administered a 2-hour IV infusion with 0.9% NaCl solution or sufficient SO-based LE (INTRALIPOS 20%) to supply 40, 100, and 200% of the basal energy requirement (BER). PMN functions were determined after collecting blood samples before, immediately after, and 24 hours after the infusion.
Results: None of the treatments significantly affected the phagocytic capacity of PMNs or circulating leukocyte numbers. The infusion providing 200% of BERs significantly reduced PMN oxidative burst activity, filamentous actin polymerization, and Cdc42 Rho guanosine triphosphatase activity immediately after its delivery. However, these functions were restored to pre-infusion values 24 hours after the infusion. The lower calorie infusions did not have these effects.
Conclusions and Clinical Importance: These results suggest that short-term infusions with a supraphysiological dose of SO-based LE may decrease the immune functions of canine PMNs. However, more long-term studies will be needed to extrapolate the effect of SO-based LE with clinically relevant doses in a practical situation.