The preliminary findings of this study were presented in abstract form as a Free Communication at the European College of Veterinary Internal Medicine Annual Forum, Munich, September 2002.
Anti-Insulin Antibodies in Diabetic Dogs Before and After Treatment with Different Insulin Preparations
Article first published online: 3 OCT 2008
Copyright © 2008 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 22, Issue 6, pages 1317–1325, November–December 2008
How to Cite
Davison, L.J., Walding, B., Herrtage, M.E. and Catchpole, B. (2008), Anti-Insulin Antibodies in Diabetic Dogs Before and After Treatment with Different Insulin Preparations. Journal of Veterinary Internal Medicine, 22: 1317–1325. doi: 10.1111/j.1939-1676.2008.0194.x
- Issue published online: 30 OCT 2008
- Article first published online: 3 OCT 2008
- Submitted March 14, 2008; Revised June 2, 2008; Accepted July 30, 2008.
- Bovine insulin;
- Glycemic control;
- Porcine insulin
Background: Anti-insulin antibodies (AIA) occur in diabetic dogs after insulin therapy, although their clinical significance is unclear.
Hypothesis: Treatment of diabetic dogs with heterologous insulin is more likely to stimulate production of AIA than is treatment with homologous insulin.
Animals: Diabetic dogs sampled before insulin therapy (n = 40), diabetic dogs sampled following treatment with porcine (homologous) insulin (n = 100), bovine (heterologous) lente insulin (n = 100), or bovine protamine zinc (PZI) insulin (n = 20), and nondiabetic control dogs (n = 120).
Methods: Prospective observational study. Sera were analyzed by ELISA for antibodies against porcine insulin, bovine insulin, insulin A, B, or C peptides, and control antigens; canine distemper virus (CDV) and canine thyroglobulin (TG). Canine isotype-specific antibodies were used to determine total and anti-insulin IgG1 : IgG2 ratios.
Results: There was no difference in CDV or TG reactivity among the groups. AIA were detected in 5 of 40 newly diagnosed (untreated) diabetic dogs. There was no significant difference in AIA (ELISA optical density reactivity) comparing control and porcine insulin-treated diabetic dogs (P > .05). Anti-insulin reactivity was most prevalent in bovine PZI insulin-treated dogs (90%; P < .01), and bovine lente insulin-treated dogs (56%; P < .01). AIA induced by treatment were enriched for the IgG1 isotype.
Conclusions and Clinical Importance: This study indicates that bovine insulin is more immunogenic than porcine insulin when used for treatment of diabetic dogs.