This study was performed at the School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
Cryopreservation of Canine Platelets
Version of Record online: 27 NOV 2008
Copyright © 2008 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 23, Issue 1, pages 138–145, January/February 2009
How to Cite
Appleman, E.H., Sachais, B.S., Patel, R., Drobatz, K.J., Groman, R.P., Kennedy, D.R., O'Donnell, P.A., Bryan, C. and Callan, M.B. (2009), Cryopreservation of Canine Platelets. Journal of Veterinary Internal Medicine, 23: 138–145. doi: 10.1111/j.1939-1676.2008.0225.x
Abstract was presented at the 2008 ACVIM Forum in San Antonio, TX.
- Issue online: 6 JAN 2009
- Version of Record online: 27 NOV 2008
- Submitted April 18, 2008; Revised June 11, 2008; Revised August 2, 2008; Accepted October 3, 2008.
Background: Platelet cryopreservation allows long-term storage and immediate availability of transfusion products.
Hypothesis: The addition of a preparation inhibiting platelet activation (Thrombosol, in 2% dimethyl sulfoxide [DMSO]) will enhance in vitro function and prolong in vivo survival of cryopreserved platelets compared with those preserved in 6% DMSO.
Animals: Thirty-three research dogs.
Methods: Prospective study. Eleven fresh canine apheresis platelet concentrates (PCs) were each split into 3 units: fresh and cryopreserved in 6% DMSO or Thrombosol. Platelet analysis, performed 1–10 weeks postfreezing, included in vitro functional testing and in vivo survival assessed by administration of biotinylated platelets.
Results: Platelet aggregation was diminished in cryopreserved PC. Cryopreserved platelets could be activated, as based on mean thrombin-stimulated P-selectin expression (6% DMSO, 23.0%; Thrombosol, 18.4%), although to a lesser extent than fresh PC (49.1%) (P < .0001). The mean maximum in vivo platelet recovery for fresh PC was 80.3%, significantly greater than recovery for 6% DMSO (49.2%) and Thrombosol PC (43.7%) (P≤ .001). The half-life (days) of fresh PC (3.8 ± 0.4) was significantly (P < .002) greater than that of 6% DMSO (1.9 ± 1.0) and Thrombosol (2.4 ± 1.1) PC, with no difference (P= .3) between cryopreserved PC.
Conclusions and Clinical Importance: Cryopreservation of canine platelets using Thrombosol did not provide any advantage over preservation using 6% DMSO. Cryopreserved platelets can be activated in vitro and provide therapeutic benefit when fresh platelets are unavailable. Further studies are needed to assess their in vivo hemostatic function.