Work was done at the Matthew J. Ryan Veterinary Hospital of the University of Pennsylvania (MJR-VHUP), School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA. Reported in part as an abstract in JVIM 21(3):595–596, 2007 (abstract # 86).
An Investigation of the Action of Neutral Protamine Hagedorn Human Analogue Insulin in Dogs with Naturally Occurring Diabetes Mellitus
Article first published online: 6 JAN 2009
Copyright © 2009 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 23, Issue 1, pages 50–55, January/February 2009
How to Cite
Palm, C.A., Boston, R.C., Refsal, K.R. and Hess, R.S. (2009), An Investigation of the Action of Neutral Protamine Hagedorn Human Analogue Insulin in Dogs with Naturally Occurring Diabetes Mellitus. Journal of Veterinary Internal Medicine, 23: 50–55. doi: 10.1111/j.1939-1676.2008.0249.x
- Issue published online: 6 JAN 2009
- Article first published online: 6 JAN 2009
- Submitted May 2, 2008; Revised July 12, 2008; Revised September 26, 2008; Accepted October 29, 2008.
- Endocrine pancreas;
Background: Neutral Protamine Hagedorn human analogue insulin (Humulin N) is commonly used for treatment of canine diabetes mellitus (DM). However, blood glucose and serum insulin concentrations in Humulin N-treated dogs with naturally occurring DM have not been reported.
Objective: To investigate blood glucose and serum insulin concentrations in the clinical setting of client-owned Humulin N-treated dogs with naturally occurring, well-regulated DM.
Animals: Ten client-owned dogs with naturally occurring, well-regulated DM.
Methods: In this clinical study, blood glucose and serum insulin concentrations were measured when dogs received food and insulin (T0), at approximately every half hour for the next 2 hours, and then approximately every 2 hours for an additional 8 hours. Insulin duration of action was defined as the number of hours from T0 to the lowest blood glucose concentration and until blood glucose concentration returned to an interpolated value of 70% of basal blood glucose concentration (Glucoseb).
Results: Mean percent of insulin-induced blood glucose suppression was 49.9 ± 17.1% (median, 46%; range, 29–78%). Insulin duration of action ranged from 4 to 10 hours. Blood glucose concentration increased initially and returned to Glucoseb within 0.6–2.2 hours after T0 in 5 dogs. This initial blood glucose surge then was followed by blood glucose suppression in all 5 dogs.
Conclusions and Clinical Importance: These results suggest that Humulin N administered SC twice daily is an effective mode of treatment for dogs with naturally occurring DM. Postprandial hyperglycemia is present in some well-regulated diabetic dogs treated with Humulin N.