This project was done at The College of Veterinary Medicine, University of Illinois at Urbana-Champaign, 1008 West Hazelwood Drive, Urbana, IL. Portions of this work were presented at the Veterinary Cancer Society Mid-Year Conference, Galena, IL, 2002.
Immunohistochemical Detection of Retinoid Receptors in Tumors from 30 Dogs Diagnosed with Cutaneous Lymphoma
Article first published online: 12 AUG 2010
Copyright © 2010 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 24, Issue 5, pages 1112–1117, September/October 2010
How to Cite
De Mello Souza, C.H., Valli, V.E.O., Selting, K.A., Kiupel, M. and Kitchell, B.E. (2010), Immunohistochemical Detection of Retinoid Receptors in Tumors from 30 Dogs Diagnosed with Cutaneous Lymphoma. Journal of Veterinary Internal Medicine, 24: 1112–1117. doi: 10.1111/j.1939-1676.2010.0571.x
- Issue published online: 2 SEP 2010
- Article first published online: 12 AUG 2010
- Submitted July 9, 2009; Revised May 21, 2010; Accepted June 21, 2010.
- Mycosis fungoides;
- Retinoic acid;
Background: Retinoids exert their effects by binding to retinoid receptors. Two types of retinoid receptors have been described: retinoic acid receptor (RAR) and retinoid X receptor (RXR), and their subtypes α, β, and γ. The expression of subtypes varies depending on the disease process. This study intended to detect the pattern of retinoid receptor expression in cutaneous lymphomas in dogs.
Hypothesis: Cutaneous lymphomas in dogs have variable expression of retinoid and retinoid X receptors.
Animals: Biopsy specimens from 30 dogs with cutaneous lymphoma.
Methods: Tissues of dogs with cutaneous lymphoma were evaluated by immunohistochemistry for expression of retinoid receptors. The tissues were tested for the presence of 3 RAR and RXR subtypes (α, β, and γ). Lymphoma immunophenotype was determined by the use of the immunohistochemical markers CD79a (B-cell) and CD3 (T-cell) in all cases.
Results: Twenty-nine of 30 dogs were CD3 positive. The retinoid receptors expressed with the greatest frequency were RARβ (87% of cases), and RXRα and RXRγ (77% of cases). The expression of RARγ was not observed.
Conclusions and Clinical Relevance: Retinoid and rexinoid receptor binding drugs may have an impact on the treatment of dogs with cutaneous lymphoma.