This work was performed at the Department of Pharmacology, Toxicology and Biochemistry and the Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Part of this work was presented as an abstract at the annual ECVIM-CA congress in Ghent, Belgium, 4–8 September 2008.
Escherichia coli Pyometra Induces Transient Glomerular and Tubular Dysfunction in Dogs
Article first published online: 12 OCT 2010
Copyright © 2010 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 24, Issue 6, pages 1263–1270, November/December 2010
How to Cite
Maddens, B., Daminet, S., Smets, P. and Meyer, E. (2010), Escherichia coli Pyometra Induces Transient Glomerular and Tubular Dysfunction in Dogs. Journal of Veterinary Internal Medicine, 24: 1263–1270. doi: 10.1111/j.1939-1676.2010.0603.x
- Issue published online: 3 NOV 2010
- Article first published online: 12 OCT 2010
- Submitted October 12, 2009; Revised July 28, 2010; Accepted August 9, 2010.
- Renal marker
Background: Pyometra in dogs has been associated with renal injury.
Hypothesis: Examine pyometra-related nephropathy by evaluating novel renal biomarkers.
Animals: Twenty-five dogs with Escherichia coli pyometra. Fourteen clinically healthy bitches of comparable age.
Methods: Prospective study. Urinary biomarkers determined by immunoassays (uIgG, uCRP, uAlb, uRBP, uTXB2) or colorimetric test (uNAG) with results normalized to urine creatinine concentration. Nonparametric Mann-Whitney U-test and Wilcoxon's signed-rank test used to compare healthy dogs and dogs with pyometra, and dogs with pyometra at initial and follow-up examination.
Results: Urinary biomarkers (median, range) significantly increased in dogs with pyometra (uIgG/Cr: 169.7 mg/g, 4.8–1052.9; uCRP/Cr: 0.260 mg/g, 0.006–3.030; uAlb/Cr: 89.5 mg/g, 8.8–832.7; uRBP/Cr: 1.66 mg/g, 0.05–21.44; uNAG/Cr: 5.8 U/g, 1.6–27.7; uTXB2/Cr: 15.3 μg/g, 3.2–139.6) compared with healthy bitches (uIgG/Cr: 3.4 mg/g, 0.6–8.9; uCRP/Cr: below detection limit; uAlb/Cr: 17.5 mg/g, 1.3–166.3; uRBP/Cr: 0.13 mg/g, 0.02–0.44; uNAG/Cr: 2.4 U/g, 1.4–7.4; uTXB2/Cr: 2.4 μg/g, 1.2–4.7) (P < .001). Six months after ovariohysterectomy, urinary biomarkers in pyometra group (uIgG/Cr: 4.7 mg/g, 1.5–99.8; uCRP/Cr: below detection limit; uAlb/Cr: 13.9 mg/g, 2.1–471.2; uRBP/Cr: 0.05 mg/g, 0.02–0.32; uNAG/Cr: 1.6 U/g, 0.9–3.3; uTXB2/Cr: 3.3 μg/g, 1.0–6.9) were significantly lower than before surgery (P < .01), and not significantly different to those of healthy dogs (P > .05).
Conclusion and Clinical Importance: Pyometra-related renal dysfunction affects the nephron both at glomerular and proximal tubular level and is a transient process in most dogs with E. coli pyometra.