Dr McNiel is presently affiliated with College of Veterinary Medicine, Michigan State University, East Lansing MI 48824. Presented in part at the 25th Annual American College of Veterinary Internal Medicine (ACVIM) Forum, Seattle, WA, 2007. The study was performed at The University of Minnesota Veterinary Medical Center, Saint Paul, Minnesota.
Evaluation of Microsatellite Instability in Urine for the Diagnosis of Transitional Cell Carcinoma of the Lower Urinary Tract in Dogs
Article first published online: 12 OCT 2010
Copyright © 2010 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 24, Issue 6, pages 1445–1451, November/December 2010
How to Cite
Sotirakopoulos, A.J., Armstrong, P.J., Heath, L., Madrill, N.J. and McNiel, E.A. (2010), Evaluation of Microsatellite Instability in Urine for the Diagnosis of Transitional Cell Carcinoma of the Lower Urinary Tract in Dogs. Journal of Veterinary Internal Medicine, 24: 1445–1451. doi: 10.1111/j.1939-1676.2010.0617.x
- Issue published online: 3 NOV 2010
- Article first published online: 12 OCT 2010
- Submitted November 27, 2009; Revised June 9, 2010; Accepted August 31, 2010.
- Cancer diagnostics;
- veterinary bladder tumor analyte
Background: The accumulation of frame-shift mutations in microsatellites (MS), termed microsatellite instability (MSI), is associated with certain tumors. MSI and its detection in urine samples has been used to aid in the detection of human bladder cancer.
Hypothesis: Evaluation of MSI in urine is a useful assay test for diagnosis of transitional cell carcinoma (TCC) in dogs and is more specific than the commercially available, veterinary bladder tumor analyte (V-BTA) test.
Animals: Seventy-three dogs: healthy controls (n= 21), proteinuric (n= 12), lower urinary tract disease excluding TCC (n= 17), and TCC (n= 23).
Methods: Prospective observational study. Urine samples collected from each animal were evaluated for MSI and using the V-BTA. For MSI detection, 22 MS sequences were polymerase chain reaction amplified from urine and blood, subjected to capillary electrophoresis, and the MS genotypes were compared. Aberration in ≥15% of MS was considered indicative of MSI.
Results: MSI was detected in 11 of 23 (48%) urine samples from dogs with TCC. MSI was also detected in 12 of 50 (24%) of the control animals, including 29, 16, and 24% of healthy, proteinuric, and lower urinary disease dogs, respectively. In this population, sensitivity and specificity of MSI analysis was 48 and 76%, respectively, compared with 83 and 64%, respectively, for the V-BTA test.
Conclusions: MS analysis as performed in this study is not useful in the diagnosis of TCC.