Biomarkers of Brain Injury in Foals with Hypoxic-Ischemic Encephalopathy
Article first published online: 8 DEC 2010
Copyright © 2010 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 25, Issue 1, pages 132–137, January/February 2011
How to Cite
Ringger, N.C., Giguère, S., Morresey, P.R., Yang, C. and Shaw, G. (2011), Biomarkers of Brain Injury in Foals with Hypoxic-Ischemic Encephalopathy. Journal of Veterinary Internal Medicine, 25: 132–137. doi: 10.1111/j.1939-1676.2010.0645.x
- Issue published online: 11 JAN 2011
- Article first published online: 8 DEC 2010
- Submitted April 6, 2010; Revised September 24, 2010; Accepted October 13, 2010.
- Phosphorylated axonal forms of neurofilament H;
- Ubiquitin C-terminal hydrolase
Background: Neonatal hypoxic-ischemic encephalopathy (NHIE) is a disease affecting newborn foals for which there is no antemortem diagnostic test.
Hypothesis: Ubiquitin C-terminal hydrolase 1 (UCHL1) and the phosphorylated axonal forms of neurofilament H (pNF-H) are markers of brain injury in foals with NHIE.
Animals: Thirty-three foals with a clinical diagnosis consistent with NHIE and 17 healthy foals.
Methods: Retrospective study. Concentrations of UCHL1 and pNF-H in plasma were measured by ELISA. The performance of the assays for the diagnosis of NHIE was assessed by receiver operating characteristic curve analysis. Concentrations of UCHL1 and pNF-H were measured throughout the brains of 2 healthy foals.
Results: The diagnostic performance of UCHL1 (AUC = 0.86) was significantly higher (P= .001) than that of pNF-H (0.52) for the diagnosis of NHIE. Median concentrations of UCHL1 (6.57 ng/mL; 2.35–11.90 ng/mL) in foals with a clinical diagnosis of NHIE were significantly (P < .001) higher than those of healthy controls (2.52 ng/mL; 1.4–4.01 ng/mL). The right sided reference interval for UCHL1 concentrations in healthy foals was 0–4.01 ng/mL. The sensitivity and specificity of UCHL1 (>4.01 ng/mL) for diagnosis of NHIE were 70% (51–84%) and 94% (72–99%), respectively. UCHL1 concentrations were higher in gray than white matter, while pNF-H concentrations were higher in white than gray matter.
Conclusions and Clinical Importance: UCHL1 has potential as a marker of brain injury in foals with NHIE.