The work was done at the University of Missouri, Columbia, MO 65211.
A Truncated Retrotransposon Disrupts the GRM1 Coding Sequence in Coton de Tulear Dogs with Bandera's Neonatal Ataxia
Version of Record online: 31 JAN 2011
Copyright © 2011 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 25, Issue 2, pages 267–272, March/April 2011
How to Cite
Zeng, R., Farias, F.H.G., Johnson, G.S., McKay, S.D., Schnabel, R.D., Decker, J.E., Taylor, J.F., Mann, C.S., Katz, M.L., Johnson, G.C., Coates, J.R. and O'Brien, D.P. (2011), A Truncated Retrotransposon Disrupts the GRM1 Coding Sequence in Coton de Tulear Dogs with Bandera's Neonatal Ataxia. Journal of Veterinary Internal Medicine, 25: 267–272. doi: 10.1111/j.1939-1676.2010.0666.x
- Issue online: 7 MAR 2011
- Version of Record online: 31 JAN 2011
- Submitted August 16, 2010; Revised October 29, 2010; Accepted November 19, 2010.
- Cerebellar ataxia;
- Genome-wide association study;
- Metabotropic glutamate receptor 1;
- Synaptic plasticity
Background: Bandera's neonatal ataxia (BNAt) is an autosomal recessive cerebellar ataxia that affects members of the Coton de Tulear dog breed.
Objective: To identify the mutation that causes BNAt.
Animals: The study involved DNA from 112 Cotons de Tulear (including 15 puppies with signs of BNAt) and 87 DNA samples from dogs of 12 other breeds.
Methods: The BNAt locus was mapped with a genome-wide association study (GWAS). The coding exons of positional candidate gene GRM1, which encodes metabotropic glutamate receptor 1, were polymerase chain reaction (PCR)-amplified and resequenced. A 3-primer PCR assay was used to genotype individual dogs for a truncated retrotransposon inserted into exon 8 of GRM1.
Results: The GWAS indicated that the BNAt locus was in a canine chromosome 1 region that contained candidate gene GRM1. Resequencing this gene from BNAt-affected puppies indicated that exon 8 was interrupted by the insertion of a 5′-truncated retrotransposon. All 15 BNAt-affected puppies were homozygous for the insert, whereas all other Cotons de Tulear were heterozygotes (n = 43) or homozygous (n = 54) for the ancestral allele. None of the 87 dogs from 12 other breeds had the insertion allele.
Conclusions and Clinical Importance: BNAt is caused by a retrotransposon inserted into exon 8 of GRM1. A DNA test for the GRM1 retrotransposon insert can be used for genetic counseling and to confirm the diagnosis of BNAt.