Dossin is presently affiliated with National Veterinary School, Toulouse cedex, France. This study was performed at the University of Pretoria, Onderstepoort, South Africa and at the University of Illinois at Urbana-Champaign, IL. Parts of the results were presented as a research abstract at the 2010 ACVIM forum, Anaheim, CA.
Effect of Parvoviral Enteritis on Plasma Citrulline Concentration in Dogs
Article first published online: 31 JAN 2011
Copyright © 2011 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 25, Issue 2, pages 215–221, March/April 2011
How to Cite
Dossin, O., Rupassara, S.I., Weng, H.-Y., Williams, D.A., Garlick, P.J. and Schoeman, J.P. (2011), Effect of Parvoviral Enteritis on Plasma Citrulline Concentration in Dogs. Journal of Veterinary Internal Medicine, 25: 215–221. doi: 10.1111/j.1939-1676.2010.0671.x
- Issue published online: 7 MAR 2011
- Article first published online: 31 JAN 2011
- Submitted July 20, 2010; Revised October 24, 2010; Accepted December 8, 2010.
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Background: Plasma citrulline concentration is a reliable marker of global enterocyte mass in humans and is markedly decreased in diffuse small intestinal diseases. However, the relationship between acute intestinal damage and plasma citrulline concentration in dogs has never been documented.
Hypothesis: That dogs with parvoviral enteritis have a lower plasma citrulline concentration than healthy dogs and that plasma citrulline concentration is a predictor of death in puppies with parvoviral enteritis.
Animals: Sixty-one dogs with spontaneous parvoviral enteritis and 14 healthy age-matched control dogs.
Methods: Observational cohort study. Plasma citrulline concentration was measured by liquid chromatography and tandem mass spectrometry in blood samples collected at admission and each day until death or discharge from the hospital. Parvovirus enteritis was confirmed by electron microscopy on a fecal sample.
Results: Median (interquartile range) plasma citrulline concentrations at admission were 2.8 μmol/L (range: 0.3, 49.0; P < .001 versus controls) in survivors (n = 49), 2.1 μmol/L (range: 0.5, 6.4, P < .001 versus controls) in nonsurvivors (n = 12) and 38.6 μmol/L (range: 11.4, 96.1) in controls (n = 14), respectively. There was no significant difference in plasma citrulline concentration between survivors and nonsurvivors within the parvovirus-infected puppies, and plasma citrulline concentration was not significantly associated with outcome in parvoviral enteritis. There were no significant changes in plasma citrulline concentration over the 8-day follow-up period.
Conclusion and Clinical Importance: Parvovirus enteritis is associated with a severe decrease in plasma citrulline concentration that does not appear to have any significant prognostic value.