The work was done at the University of Liverpool.
Leptin Expression in Dogs with Cardiac Disease and Congestive Heart Failure
Article first published online: 30 AUG 2011
Copyright © 2011 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 25, Issue 5, pages 1017–1024, September/October 2011
Total views since publication: 19
How to Cite
Fonfara, S., Hetzel, U., Tew, S.R., Dukes-Mcewan, J., Cripps, P. and Clegg, P.D. (2011), Leptin Expression in Dogs with Cardiac Disease and Congestive Heart Failure. Journal of Veterinary Internal Medicine, 25: 1017–1024. doi: 10.1111/j.1939-1676.2011.00782.x
The study was not supported. Parts of the study were presented at the 2011 ACVIM Forum, Denver, CO.
- Issue published online: 20 SEP 2011
- Article first published online: 30 AUG 2011
- Manuscript Accepted: 6 JUL 2011
- Manuscript Revised: 4 JUN 2011
- Manuscript Received: 23 DEC 2010
- Body condition score;
- Real-time PCR;
Leptin belongs to the group of adipokines and has recently attracted attention because of its effects on the cardiovascular system. Increased leptin concentrations are reported in obese dogs but its role in cardiac disease (CD) is not known. Therefore, we investigated leptin expression in blood samples from dogs with congestive heart failure (CHF), and from myocardial samples of dogs with CDs.
Leptin mRNA was analyzed from blood samples of 8 dogs presented for cardiac screening in which no abnormalities were detected and 8 dogs in CHF. In addition, myocardial samples (interventricular septum, right and left atria, and ventricles) of 10 dogs with no cardiac abnormalities (controls), 7 dogs with acquired and 3 dogs with congenital CDs were investigated using real-time polymerase chain reaction (PCR).
Dogs with CHF had significantly higher blood concentrations of leptin mRNA than dogs without CD (P = .013). Myocardial leptin expression was significantly increased in acquired (P = .035) and decreased in congenital CD (P = .016) in comparison to controls. Dogs in heart failure stage D showed higher myocardial leptin concentrations than dogs in stage C3 and B (P = .031). Differences according to myocardial region (P < .05) were detected and higher leptin concentrations were present in the atria in comparison to the ventricles in dogs with CD (P = .005). Comparing male and female dogs with CD revealed higher leptin concentrations in female dogs (P = .001).
These results indicate leptin mRNA concentrations vary with CD, severity of CD, myocardial region, and possibly sex. Therefore, leptin might play a role in canine CD.