Resting Energy Expenditure per Lean Body Mass Determined by Indirect Calorimetry and Bioelectrical Impedance Analysis in Cats
Article first published online: 1 NOV 2011
Copyright © 2011 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 25, Issue 6, pages 1341–1350, November-December 2011
How to Cite
Center, S.A., Warner, K.L., Randolph, J.F., Wakshlag, J.J. and Sunvold, G.D. (2011), Resting Energy Expenditure per Lean Body Mass Determined by Indirect Calorimetry and Bioelectrical Impedance Analysis in Cats. Journal of Veterinary Internal Medicine, 25: 1341–1350. doi: 10.1111/j.1939-1676.2011.00824.x
- Issue published online: 16 NOV 2011
- Article first published online: 1 NOV 2011
- Manuscript Accepted: 15 SEP 2011
- Manuscript Revised: 25 JUN 2011
- Manuscript Received: 27 OCT 2010
- Cornell Feline Health Center Research Grant Program
- Iams Pet Food Company
- Body condition score;
- Doubly labeled water;
Resting energy expenditure (REE) approximates ≥60% of daily energy expenditure (DEE). Accurate REE determination could facilitate sequential comparisons among patients and diseases if normalized against lean body mass (LBM).
(1) Validate open-flow indirect calorimetry (IC) system and multifrequency bioelectrical impedance analysis (MF-BIA) to determine REE and LBM, respectively, in healthy nonsedated cats of varied body conditions; (2) normalize REE against LBM.
Fifty-seven adult neutered domestic short-haired cats with stable BW.
Continuous (45-min) IC-measurements determined least observed metabolism REE. Cage gas flow regulated with mass flow controllers was verified using nitrogen dilution; span gases calibrated gas measurements. Respiratory quotient accuracy was verified using alcohol combustion. IC-REE was compared to DEE, determined using doubly labeled water. MF-BIA LBM was validated against criterion references (deuterium, sodium bromide). Intra- and interassay variation was determined for IC and MF-BIA.
Mean IC-REE (175 ± 38.7 kcal; 1.5–14% intra- and interassay CV%) represented 61 ± 14.3% of DEE. Best MF-BIA measurements were collected in sternal recumbency and with electrodes in neck-tail configuration. MF-BIA LBM was not significantly different from criterion references and generated LBM interassay CV% of 6.6–10.1%. Over- and underconditioned cats had significantly (P ≤ .05) lower and higher IC-REE (kcal/kg) respectively, compared with normal-conditioned cats. However, differences resolved with REE/LBM (approximating 53 ± 10.3 kcal/LBM [kg]).
Conclusions and Clinical Importance
IC and MF-BIA validated herein reasonably estimate REE and LBM in cats. REE/LBM(kg) may permit comparison of energy utilization in sequential studies or among different cats.