This work was performed at the North Carolina State College of Veterinary Medicine Veterinary Teaching Hospital.
Collection of Peripheral Blood CD34+ Progenitor Cells from Healthy Dogs and Dogs Diagnosed with Lymphoproliferative Diseases Using a Baxter-Fenwal CS-3000 Plus Blood Cell Separator
Article first published online: 16 NOV 2011
Copyright © 2011 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 25, Issue 6, pages 1406–1413, November-December 2011
How to Cite
Suter, S.E. (2011), Collection of Peripheral Blood CD34+ Progenitor Cells from Healthy Dogs and Dogs Diagnosed with Lymphoproliferative Diseases Using a Baxter-Fenwal CS-3000 Plus Blood Cell Separator. Journal of Veterinary Internal Medicine, 25: 1406–1413. doi: 10.1111/j.1939-1676.2011.00827.x
- Issue published online: 16 NOV 2011
- Article first published online: 16 NOV 2011
- Manuscript Accepted: 19 SEP 2011
- Manuscript Revised: 26 JUL 2011
- Manuscript Received: 30 MAR 2011
- Hematopoietic cell transplantation;
- Mononuclear cells
Canine peripheral blood mononuclear cell (PBMC) apheresis using a Baxter-Fenwal CS-3000 Plus automated blood cell separator has not been reported.
To determine the feasibility and safety of using a CS-3000 Plus blood cell separator with a small volume separation container holder (SVSCH) and small volume collection chamber (SVCC) to harvest canine PBMCs from dogs weighing <50 kg.
Eight healthy mongrel dogs and 11 client-owned dogs in clinical remission for lymphoproliferative diseases (LPD).
In this prospective study, aphereses were performed using a Baxter-Fenwal CS-3000 Plus blood cell separator, with or without recombinant human granulocyte colony-stimulating factor (rhG-CSF) treatment.
Aphereses from 6 healthy dogs given rhG-CSF yielded an average of 1.1 × 107 ± 8.2 × 106CD34+ cells/kg. Aphereses from LPD dogs given rhG-CSF yielded an average of 5.4 × 106 ± 3.25 × 106CD34+ cells/kg (P = .17). Higher hematocrit in both groups of dogs receiving rhG-CSF correlated with an increased number of CD34+ cells/kg harvested (healthy, P = .04; LPD, P = .05). Apheresis was well tolerated by all dogs.
Conclusions and Clinical Importance
Canine PBMC apheresis using the Baxter-Fenwal CS-3000 Plus cell separator with an SVSCH and SVCC is a feasible and safe option for harvesting an adequate number of CD34+ peripheral blood progenitor cells from dogs weighing ≥17 kg for hematopoietic cell transplantation.