• Open Access

Collection of Peripheral Blood CD34+ Progenitor Cells from Healthy Dogs and Dogs Diagnosed with Lymphoproliferative Diseases Using a Baxter-Fenwal CS-3000 Plus Blood Cell Separator


  • S.E. Suter

    Corresponding author
    1. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC
    2. Lineberger Comprehensive Cancer Center, Chapel Hill, NC
    • Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC
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  • This work was performed at the North Carolina State College of Veterinary Medicine Veterinary Teaching Hospital.

Corresponding author: S.E. Suter, Department of Clinical Sciences College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, CVM Research #308, Raleigh, NC 27606; e-mail:steven_suter@ncsu.edu



Canine peripheral blood mononuclear cell (PBMC) apheresis using a Baxter-Fenwal CS-3000 Plus automated blood cell separator has not been reported.


To determine the feasibility and safety of using a CS-3000 Plus blood cell separator with a small volume separation container holder (SVSCH) and small volume collection chamber (SVCC) to harvest canine PBMCs from dogs weighing <50 kg.


Eight healthy mongrel dogs and 11 client-owned dogs in clinical remission for lymphoproliferative diseases (LPD).


In this prospective study, aphereses were performed using a Baxter-Fenwal CS-3000 Plus blood cell separator, with or without recombinant human granulocyte colony-stimulating factor (rhG-CSF) treatment.


Aphereses from 6 healthy dogs given rhG-CSF yielded an average of 1.1 × 107 ± 8.2 × 106CD34+ cells/kg. Aphereses from LPD dogs given rhG-CSF yielded an average of 5.4 × 106 ± 3.25 × 106CD34+ cells/kg (= .17). Higher hematocrit in both groups of dogs receiving rhG-CSF correlated with an increased number of CD34+ cells/kg harvested (healthy, = .04; LPD, = .05). Apheresis was well tolerated by all dogs.

Conclusions and Clinical Importance

Canine PBMC apheresis using the Baxter-Fenwal CS-3000 Plus cell separator with an SVSCH and SVCC is a feasible and safe option for harvesting an adequate number of CD34+ peripheral blood progenitor cells from dogs weighing ≥17 kg for hematopoietic cell transplantation.