The work was done and supported by funds at the University of Calgary, Faculty of Veterinary Medicine (UCVM).
Comparison of Cytokine mRNA Expression in the Bronchoalveolar Lavage Fluid of Horses with Inflammatory Airway Disease and Bronchoalveolar Lavage Mastocytosis or Neutrophilia Using REST Software Analysis
Article first published online: 14 DEC 2011
Copyright © 2011 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 26, Issue 1, pages 153–161, January-February 2012
How to Cite
Beekman, L., Tohver, T. and Léguillette, R. (2012), Comparison of Cytokine mRNA Expression in the Bronchoalveolar Lavage Fluid of Horses with Inflammatory Airway Disease and Bronchoalveolar Lavage Mastocytosis or Neutrophilia Using REST Software Analysis. Journal of Veterinary Internal Medicine, 26: 153–161. doi: 10.1111/j.1939-1676.2011.00847.x
The study was presented at the 2011 ACVIM Forum, Denver, CO.
- Issue published online: 10 JAN 2012
- Article first published online: 14 DEC 2011
- Manuscript Accepted: 25 OCT 2011
- Manuscript Revised: 1 AUG 2011
- Manuscript Received: 1 APR 2011
- Gene expression;
- Polymerase chain reaction;
The pathophysiology of inflammatory airway disease (IAD) is unknown, but in some cases involves the accumulation of mast cells, neutrophils, or both in the bronchoalveolar lavage fluid (BALF). The objective of this study was to characterize cytokine gene expression in the BALF cells of horses with IAD, including a comparison of cytokine gene expression between IAD horses with increased BALF mast cells (IAD-Mast) or neutrophils (IAD-Neutro).
The mRNA expression of IL-4, IFN-γ, IL-17, IL-8, IL-1β, IL-5, IL-6, IL-10, IL-12p35, and eotaxin-2 was studied by quantitative polymerase chain reaction (QPCR) with efficiency correction in BALF samples of 17 horses with IAD (IAD-total), also subcategorized as 8 IAD-Mast and 9 IAD-Neutro, and 10 controls. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a reference gene. Relative expression software tool (REST) analysis provided ratios of expression, statistical analysis, and confidence intervals for the results.
Compared with the control group, IL-5, IL-1β, IL-6, IL-8, and IL-10 mRNA expression was upregulated 3.5-, 3.4-, 2.8-, 2.2-, and 1.9-fold, respectively, in the IAD-total group. The IAD-Neutro group showed increased expression of IL-17, IL-8, and IL-5 (4.7-, 2.5-, and 2.9-fold, respectively) and a decreased expression of IL-4 (3.4-fold) compared with the IAD-Mast group.
Cytokines from the Th2 family plays a key role in IAD and a different pathophysiology may be involved in mast cell versus neutrophil BALF accumulation in IAD horses.