This study was performed primarily at the Cornell University, College of Veterinary Medicine. PCR and MAT were also performed at IDEXX Laboratories in Sacramento, CA and North Grafton, MA, respectively. The study was partially supported by a grant from IDEXX Laboratories, and was presented in poster form at the 2010 ACVIM Forum, Anaheim, CA.
Effects of Recent Leptospira Vaccination on Whole Blood Real-Time PCR Testing in Healthy Client-Owned Dogs
Article first published online: 19 DEC 2011
Copyright © 2011 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 26, Issue 1, pages 149–152, January-February 2012
How to Cite
Midence, J.N., Leutenegger, C.M., Chandler, A.M. and Goldstein, R.E. (2012), Effects of Recent Leptospira Vaccination on Whole Blood Real-Time PCR Testing in Healthy Client-Owned Dogs. Journal of Veterinary Internal Medicine, 26: 149–152. doi: 10.1111/j.1939-1676.2011.00852.x
- Issue published online: 10 JAN 2012
- Article first published online: 19 DEC 2011
- Manuscript Accepted: 11 NOV 2011
- Manuscript Revised: 5 OCT 2011
- Manuscript Received: 18 JUL 2011
- Canine leptospirosis;
- Real-time PCR;
Bacterin-based canine Leptospira vaccines could present a challenge for the use of whole blood real-time polymerase chain reaction (PCR) as a diagnostic tool. Recent vaccination could induce positive results if the targeted DNA fragment is present within the vaccine and in the blood of the recently vaccinated dog.
The objective of this study was to assess whether 2 available 4-serovar vaccines induce a positive real-time PCR reaction in the blood of healthy recently vaccinated dogs.
Twenty healthy dogs.
This was a prospective study. Dogs were assigned to 1 of 2 vaccine groups. Both vaccines were culture-based and include Leptospira interrogans serovars Pomona, Canicola, and Icterohaemorrhagiae and Leptospira kirschneri serovar Grippotyphosa. Whole blood for real-time PCR and serum for the microscopic agglutination test (MAT) were collected prior to and 3 and 7 days after vaccination and weekly thereafter for 8 weeks. Two real-time PCR tests targeting 2 different genes were performed independently in a blinded fashion.
Both Leptospira vaccines produced positive real-time PCR reactions when assayed undiluted or diluted 1 : 100 in canine blood. However, blood samples drawn from all dogs at all time points after vaccination were negative on PCR. All dogs developed MAT titers.
Conclusions and Clinical Importance
Recent vaccination with 2 commercially available vaccines does not interfere with the use of real-time PCR for the identification of acute Leptospira infection in dogs.