Concentrations of Plasma Metabolites, Hormones, and mRNA Abundance of Adipose Leptin and Hormone-Sensitive Lipase in Ketotic and Nonketotic Dairy Cows
Article first published online: 1 FEB 2012
Copyright © 2012 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 26, Issue 2, pages 415–417, March-April 2012
How to Cite
Xia, C., Wang, Z., Xu, C. and Zhang, H.Y. (2012), Concentrations of Plasma Metabolites, Hormones, and mRNA Abundance of Adipose Leptin and Hormone-Sensitive Lipase in Ketotic and Nonketotic Dairy Cows. Journal of Veterinary Internal Medicine, 26: 415–417. doi: 10.1111/j.1939-1676.2011.00863.x
- Issue published online: 20 MAR 2012
- Article first published online: 1 FEB 2012
- Manuscript Accepted: 29 NOV 2011
- Manuscript Revised: 24 OCT 2011
- Manuscript Received: 24 APR 2011
- Chinese National Nature Science Foundation. Grant Number: 30840060
- Agricultural Reclamation Head Office of Heilongjiang Province. Grant Number: HNKXIV-08-01-07
- Biochemical parameters;
- Gene expression;
Ketosis is an important metabolic disorder of dairy cows during the transition period. There have been many reports on the etiology of ketosis in periparturient cows, but little is known about its molecular etiology.
The objective of this study was to clarify the status of fat mobilization and mRNA abundance of leptin and hormone-sensitive lipase in cows with spontaneous clinical ketosis.
Ten ketotic Holstein cows and 10 nonketotic Holstein cows were used as the experimental animals.
Six blood biochemical parameters were evaluated by means of individual analysis method for 2 groups of cows. The mRNA abundance of leptin and hormone-sensitive lipase in tail fat tissue from 2 groups of cows was measured by real-time (RT)-PCR, with a fluorescent Taqman probe and a standard curve.
The plasma concentrations of glucose (P = 0.01), and leptin (P = 0.03), insulin (P = 0.05), and the ratio of insulin to glucagon (P = 0.04) were lower in ketotic compared with nonketotic cows, whereas there were marked increases in the plasma concentrations of nonesterified fatty acid and β-hydroxybutyric acid (P = 0.005). The mRNA abundance of leptin (P = 0.04) and hormone-sensitive lipase (P = 0.02) in the fat tissue of ketotic cows was lower relative to that of nonketotic cows.
Conclusions and Clinical Importance
The ketotic cows showed characteristics of type I ketosis and some adaptive changes to negative energy balance in the plasma leptin concentration and mRNA abundance of fat leptin and hormone-sensitive lipase.