This work was done in The Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University.
Effects of Prostaglandin E1 on the Preparation of Platelet Concentrates in Dogs
Article first published online: 23 JAN 2012
Copyright © 2012 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 26, Issue 2, pages 370–376, March-April 2012
How to Cite
Segawa, K., Kondo, T., Kimura, S., Fujimoto, A., Kato, T., Ishikawa, T., Neo, S., Hisasue, M., Yamada, T. and Tsuchiya, R. (2012), Effects of Prostaglandin E1 on the Preparation of Platelet Concentrates in Dogs. Journal of Veterinary Internal Medicine, 26: 370–376. doi: 10.1111/j.1939-1676.2011.00881.x
Previously presented at the 150th Meeting of the Japanese Society of Veterinary Science, 2010.
- Issue published online: 20 MAR 2012
- Article first published online: 23 JAN 2012
- Manuscript Accepted: 16 DEC 2011
- Manuscript Revised: 20 NOV 2011
- Manuscript Received: 30 MAY 2011
- The Promotion and Mutual Aid Corporation for Private Schools of Japan
Platelet concentrates (PC) are prepared by centrifugation of platelet-rich plasma (PRP) that is prepared by centrifugation of whole blood. The resuspension of the platelet pellet during PC preparation from dogs is difficult because of platelet activation induced by centrifugation.
To investigate the efficacy of adding prostaglandin E1 (PGE1) to prevent platelet activation during PC preparation from dogs.
Fifteen healthy Beagle dogs.
Prospective, experimental trial: PGE1 was added to PRP before the high-speed centrifugation during PC preparation. To estimate the effect of this addition, we assessed the platelet aggregability before transfusion, the survival of the platelets after transfusion, and the platelet reactivity after transfusion, which is estimated by the P-selectin expression of the platelets when stimulated by thrombin.
The difficulty associated with platelet resuspension was resolved by PGE1.PGE1 strongly inhibited platelet aggregation induced by collagen and ADP; however, it recovered after the platelets were resuspended in plasma without PGE1 (mean aggregation ratio; collagen: 10.00–80.80%, ADP: 8.20–53.60%). Survival of the platelets after transfusion was not affected by PGE1 (mean 8.04 and 7.56 days, without and with PGE1), and thrombin-induced P-selectin expression after transfusion in PGE1-treated PC was also well maintained (mean positive ratio 53.7 and 47.9%, before and 24 hours after transfusion).
Conclusions and Clinical Importance
The addition of PGE1 in PRP before the centrifugation of PRP can improve the preparation efficiency of PC from dogs, while maintaining the therapeutic efficacy of the platelets.