Comparison of IV and IM Formulations of Synthetic ACTH for ACTH Stimulation Tests in Healthy Dogs
Article first published online: 23 JAN 2012
Copyright © 2012 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 26, Issue 2, pages 412–414, March-April 2012
How to Cite
Cohen, T.A. and Feldman, E.C. (2012), Comparison of IV and IM Formulations of Synthetic ACTH for ACTH Stimulation Tests in Healthy Dogs. Journal of Veterinary Internal Medicine, 26: 412–414. doi: 10.1111/j.1939-1676.2011.00882.x
- Issue published online: 20 MAR 2012
- Article first published online: 23 JAN 2012
- Manuscript Accepted: 13 DEC 2011
- Manuscript Revised: 8 NOV 2011
- Manuscript Received: 15 AUG 2011
- Adrenal gland;
Two commercially available forms of synthetic ACTH are used to diagnose and monitor hyper- and hypoadrenocorticism in dogs.
To compare the biologic activity of the liquid and lyophilized forms of cosyntropin.
Eighteen privately owned healthy dogs were included.
Dogs were assigned to one of 2 groups of 9 dogs each. Group 1 dogs were tested with the lyophilized product first and the liquid solution 30–60 days later. The Group 2 dogs were tested with the liquid solution first and the lyophilized drug 30–60 days later. For the ACTH stimulation tests, serum samples were collected before and 1 hour after IM administration of 0.25 mg reconstituted lyophilized product or 1 hour after IV administration of 0.25 mg of liquid solution. Cortisol concentrations of all serum samples were measured by use of a commercial cortisol radioimmunoassay.
Serum cortisol concentrations before and after ACTH stimulation did not differ significantly between groups (P = .57). In addition, no individual dog had as much as a 20% difference in serum cortisol concentrations after administration of either ACTH formulation.
Conclusions and Clinical Importance
Given the lack of significant differences of the ACTH stimulation test results, the lyophilized and liquid solution products can be used interchangeably.