jvim891-sup-0001-FigS1.pdfapplication/PDF402KFig S1. Plot of urinary NAG activity during storage at room temperature over 24 hours. A minimal but statistically significant decrease in NAG activity was observed after 12–24 hours for 2 samples (represented by the orange and green lines).
jvim891-sup-0002-FigS2.pdfimage/eps402KFig S2. Plots of urinary NAG activity during storage at 4, −20, and −80°C over 1 year. A statistically significant, but not clinically significant, increase in NAG activity was observed for 2 of the samples stored at 4°C (represented by the green and blue lines). There was no statistical evidence of an effect of storage temperature on NAG activity for 1 year at −20 or −80°C.
jvim891-sup-0003-FigS3.pdfimage/eps402KFig S3. Plot of urinary NAG activity and NGAL concentration over 0–5 and 0–4 freeze-thaw cycles, respectively. There was a statistically significant (P = .05) but clinically insignificant increase in NAG activity after 4 freeze-thaw cycles. The NGAL concentration was not significantly affected with up to 4 freeze-thaw cycles.
jvim891-sup-0004-FigS4.pdfimage/eps407KFig S4. Descriptive plots (median, range) and linear regression plots (mean [B2M, NGAL, NAG, IgG] or median [RBP], confidence interval) for each analyte based on the year in which the urine sample was collected. Although all analytes except for NAG exhibited an increasing trend as storage time decreased, a statistically significant increase was not observed. The following P-values were obtained: uRBP/c = 0.072; uB2M = 0.065; uNGAL/c = 0.065; uNAG/c = 0.624; uIgG/c = 0.062.
jvim891-sup-0005-TableS1.pdfimage/eps407KTable S1. Correlations (Corr) of urinary biomarkers in dogs with XLHN. For all correlations P < .001.

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