Dr DeClue and Dr Sharp share the first author position.
Plasma Inflammatory Mediator Concentrations at ICU Admission in Dogs with Naturally Developing Sepsis
Article first published online: 7 MAR 2012
Copyright © 2012 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 26, Issue 3, pages 624–630, May-June 2012
How to Cite
DeClue, A.E., Sharp, C.R. and Harmon, M. (2012), Plasma Inflammatory Mediator Concentrations at ICU Admission in Dogs with Naturally Developing Sepsis. Journal of Veterinary Internal Medicine, 26: 624–630. doi: 10.1111/j.1939-1676.2012.00895.x
- Issue published online: 2 MAY 2012
- Article first published online: 7 MAR 2012
- Manuscript Accepted: 19 JAN 2012
- Manuscript Revised: 1 NOV 2011
- Manuscript Received: 18 AUG 2011
- University of Missouri Research Council
- Systemic inflammatory response syndrome
Identifying biomarkers to aide in the diagnosis and prognostication of sepsis in dogs would be valuable to veterinarians.
To compare plasma inflammatory mediator concentrations among dogs with sepsis, noninfectious systemic inflammatory response syndrome (NSIRS), and healthy dogs.
Dogs with sepsis (n = 22), NSIRS (n = 23), and healthy dogs (n = 13) presenting to the intensive care unit (ICU) at a veterinary teaching hospital.
Prospective observational study. Clinical parameters were recorded for each dog and plasma tumor necrosis factor (TNF) bioactivity and concentrations of interleukin (IL)-6, CXC chemokine ligand (CXCL)-8 and IL-10 were determined at ICU presentation.
Dogs with sepsis and NSIRS were significantly more likely to have measurable TNF activity (sepsis 20/22; NSIRS 19/20; healthy 0/13) and IL-6 concentration (sepsis 12/22; NSIRS 15/23; healthy 2/13), than healthy dogs. Healthy dogs (9/13) were significantly more likely to have measurable plasma IL-10 concentrations than dogs with sepsis (4/19), but not NSIRS (7/20). None of the inflammatory mediators evaluated had optimal sensitivity or specificity for the diagnosis of sepsis. Twelve of 22 dogs with sepsis and 15/23 dogs with NSIRS survived to discharge; none of the measured biomarkers correlated with survival to discharge.
Conclusions and Clinical Importance
Sepsis and NSIRS are associated with increased production of the proinflammatory cytokines TNF and IL-6. In addition, sepsis is associated with decreased production of the anti-inflammatory cytokine IL-10. Despite this, plasma TNF, IL-6, CXCL-8, and IL-10 measured at ICU presentation do not appear to be valuable biomarkers to differentiate sepsis from NSIRS, or predict hospital outcome.