The abstract was presented at the 2010 ACVIM Forum, Denver, CO.
Association of Adrenocorticotrophin and Cortisol Concentrations with Peripheral Blood Leukocyte Cytokine Gene Expression in Septic and Nonseptic Neonatal Foals
Article first published online: 23 MAR 2012
Copyright © 2012 by the American College of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine
Volume 26, Issue 3, pages 654–661, May-June 2012
How to Cite
Gold, J.R., Cohen, N.D. and Welsh, T.H. (2012), Association of Adrenocorticotrophin and Cortisol Concentrations with Peripheral Blood Leukocyte Cytokine Gene Expression in Septic and Nonseptic Neonatal Foals. Journal of Veterinary Internal Medicine, 26: 654–661. doi: 10.1111/j.1939-1676.2012.00910.x
- Issue published online: 2 MAY 2012
- Article first published online: 23 MAR 2012
- Manuscript Accepted: 13 FEB 2012
- Manuscript Revised: 26 JAN 2012
- Manuscript Received: 15 MAY 2011
- Gene expression;
The hypothalamic-pituitary-adrenal (HPA) is influenced by the proinflammatory cytokines IL-6, IL-1β, and TNF-α in critically ill humans. Information about the association of cytokines with the HPA axis in neonatal foals is lacking.
The objectives were to describe for hospitalized septic and nonseptic foals (1) temporal changes in blood concentrations of ACTH, and cortisol, and leukocyte cytokine gene expression, and (2) coassociation of these HPA axis hormones with blood leukocyte cytokine gene expression.
Hospitalized septic foals (N = 15) and hospitalized nonseptic foals (N = 11).
Blood samples, obtained from study foals at admission (T = 0), and 24 (T = 1), 48 (T = 2), 72 (T = 3), and 96 (T = 4) hours after admission, were processed to isolate RNA from leukocytes and to harvest plasma and serum for hormone assays. Plasma ACTH and serum cortisol concentrations were determined by radioimmunoassay. Leukocyte mRNA expression of IL-1β IL-6, IL-8, IL-10, and TNF-α was determined using RT-PCR.
Cortisol concentrations were greater (P < .05) in foals at admission than at other time points. The expressions of IL-8 and IL-10 mRNA were lower (P < .05) at each time point in septic than in nonseptic foals. Among septic foals, ACTH was positively associated (P = .0026) with IL-6 mRNA expression.
Sepsis influences secretion of the HPA axis hormones and expression of cytokines in foals. A positive association with the HPA axis and IL-6 expression was detected. The clinical importance of these findings requires additional study.