Congenital Hypothyroidism with Goiter in Tenterfield Terriers

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Filename	Format	Size	Description
jvim1015-sup-0001-FigureS1.tif	image/tif	5889K	Figure S1. Photomicrograph of thyroid tissue from a 5-week-old Tenterfield Terrier exhibiting CHG. There is diffuse follicular epithelial cell hyperplasia. Follicle spaces are largely obliterated by cuboidal to columnar epithelial cells piled up as blunt papillae and exhibiting multiple small cytoplasmic vacuoles. The remaining colloid spaces are small and have scant, poorly staining colloid. The bar indicates 200 μm.
jvim1015-sup-0002-FigureS2.tif	image/tif	19540K	Figure S2. Local structure modeling demonstrating the predicted effect of the Tenterfield Terrier mutation in TPO. The structure of human myeloperoxidase, a TPO homologue, determined from high-resolution x-ray crystallographic data is illustrated herein as a discontinuous ribbon diagram using the PyMOL Molecular Graphics System. A star denotes the residue homologous to R593 which is replaced by tryptophan in CHG-affected dogs. The hydrogen bonds formed between the residues homologous to R593, aspartate 642, and tyrosine 637 in canine TPO are shown as dashed lines. Disruption of this hydrogen bonding sequence likely interferes with the maintenance of local protein structure necessary for arginine 591 to coordinate the heme group (in red), a key interaction required for TPO catalytic activity.

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