Expression of wild-type and mutant S20G hIAPP in physiologic knock-in mouse models fails to induce islet amyloid formation, but induces mild glucose intolerance
Article first published online: 11 OCT 2011
© 2011 Asian Association for the Study of Diabetes and Blackwell Publishing Asia Pty Ltd
Journal of Diabetes Investigation
Volume 3, Issue 2, pages 138–147, April 2012
How to Cite
Hiddinga, H. J., Sakagashira, S., Ishigame, M., Madde, P., Sanke, T., Nanjo, K., Kudva, Y. C., Lee, J. J., van Deursen, J. and Eberhardt, N. L. (2012), Expression of wild-type and mutant S20G hIAPP in physiologic knock-in mouse models fails to induce islet amyloid formation, but induces mild glucose intolerance. Journal of Diabetes Investigation, 3: 138–147. doi: 10.1111/j.2040-1124.2011.00166.x
- Issue published online: 27 MAR 2012
- Article first published online: 11 OCT 2011
- Received 2 December 2010; revised 27 July 2011; accepted 9 August 2011
Appendix S1 Materials and Methods, providing detailed description of vector construction for the homologous replacement of the mouse IAPP gene for the wild-type and S20G mutant hIAPP in mouse embryonic stem cells and subsequent generation of the wild-type and S20G mutant hIAPP knock-in mice.
Appendix S2 Intraislet amyloidogenesis in WW and GW chimaeric 129Sv/E founder mice at 15 months.
|JDI_166_sm_Appendix-S1-2.pdf||4523K||Supporting info item|
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