These authors contributed equally to this work.
Ingestion of a moderate high-sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon-like peptide-1 secretion
Article first published online: 20 MAR 2012
© 2012 Asian Association for the Study of Diabetes and Wiley Publishing Asia Pty Ltd
Journal of Diabetes Investigation
Volume 3, Issue 5, pages 432–440, October 2012
How to Cite
Sakamoto, E., Seino, Y., Fukami, A., Mizutani, N., Tsunekawa, S., Ishikawa, K., Ogata, H., Uenishi, E., Kamiya, H., Hamada, Y., Sato, H., Harada, N., Toyoda, Y., Miwa, I., Nakamura, J., Inagaki, N., Oiso, Y. and Ozaki, N. (2012), Ingestion of a moderate high-sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon-like peptide-1 secretion. Journal of Diabetes Investigation, 3: 432–440. doi: 10.1111/j.2040-1124.2012.00208.x
- Issue published online: 18 OCT 2012
- Article first published online: 20 MAR 2012
- Received 31 August 2011; revised 30 January 2012; accepted 30 January 2012
- Glucagon-like peptide-1;
- High-sucrose diet
Aims/Introduction: Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high-sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried out using diets with much higher sucrose contents (50–70% of the total calories) than are typically ingested by humans. In the present study, we examined the effects of a moderate SUC on glucose metabolism and the underlying mechanism.
Materials and Methods: C57BL/6J mice received a SUC (38.5% sucrose), a high-starch diet (ST) or a control diet for 5 weeks. We assessed glucose tolerance, incretin secretion and liver glucose metabolism.
Results: An oral glucose tolerance test (OGTT) showed that plasma glucose levels in the early phase were significantly higher in SUC-fed mice than in ST-fed or control mice, with no change in plasma insulin levels at any stage. SUC-fed mice showed a significant improvement in insulin sensitivity. Glucagon-like peptide-1 (GLP-1) secretion 15 min after oral glucose administration was significantly lower in SUC-fed mice than in ST-fed or control mice. Hepatic glucokinase (GCK) activity was significantly reduced in SUC-fed mice. During the OGTT, the accumulation of glycogen in the liver was suppressed in SUC-fed mice in a time-dependent manner.
Conclusions: These results indicate that mice that consume a moderate SUC show glucose intolerance with a reduction in hepatic GCK activity and impairment in GLP-1 secretion. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2012.00208.x, 2012)