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CcpA regulates biofilm formation and competence in Streptococcus gordonii

Authors

  • L. Zheng,

    1.  Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    2.  Department of Microbiology and Parasitology, China Medical University, Shenyang City, Liaoning Province, China
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  • Z. Chen,

    1.  Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
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  • A. Itzek,

    1.  Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
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  • M.C. Herzberg,

    1.  Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, MN, USA
    2.  Mucosal and Vaccine Research Center, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN, USA
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  • J. Kreth

    1.  Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    2.  College of Dentistry, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
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Jens Kreth, Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, BMSB 907, 940 Stanton L. Young Boulevard, Oklahoma City, OK 73104, USA. Tel.: +1 405 271 1202; fax: +1 405 271 3117; E-mail: jkreth@ouhsc.edu

Summary

Streptococcus gordonii is an important member of the oral biofilm community. As an oral commensal streptococcus, S. gordonii is considered beneficial in promoting biofilm homeostasis. CcpA is known as the central regulator of carbon catabolite repression in Gram-positive bacteria and is also involved in the control of virulence gene expression. To further establish the role of CcpA as central regulator in S. gordonii, the effect of CcpA on biofilm formation and natural competence of S. gordonii was investigated. These phenotypic traits have been suggested to be important to oral streptococci in coping with environmental stress. Here we demonstrate that a CcpA mutant was severely impaired in its biofilm-forming ability, showed a defect in extracellular polysaccharide production and reduced competence. The data suggest that CcpA is involved in the regulation of biofilm formation and competence development in S. gordonii.

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