Cellular sources of proteolytic enzymes in equine joints

Authors

  • SUE SPIERS,

    1. Divisions of Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
    2. Equine Studies and Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
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    • *

      Molecular Genetics, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.

  • S. A. MAY,

    1. Divisions of Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
    2. Equine Studies and Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
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    • Department of Large Animal Medicine and Surgery, Royal Veterinary College, Hawkshead House, North Mymms, Hafield, Herts AL9 7TA, UK.

  • D. BENNETT,

    1. Divisions of Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
    2. Small Animal Studies, Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
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  • G. B. EDWARDS

    1. Divisions of Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
    2. Equine Studies and Department of veterinary Clinical Science, University of Liverpool, Leahurst, Neston, South Wirral L64 7TE, UK.
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Summary

Isolated equine blood and articular cells were investigated for proteolytic enzyme production by means of gel filtration and analysis on 14C-acetylated collagen and casein substrates. Significant amounts of collagenase and caseinase activity were produced by cultured synoviocytes stimulated with equine interleukin 1, although large amounts of collagenase also originated from neutrophils.

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