Investigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds
Article first published online: 10 JUN 2010
© 2006 EVJ Ltd
Equine Veterinary Journal
Special Issue: Proceedings of the 7th International Conference of Equine Exercise Physiology
Volume 38, Issue S36, pages 239–244, August 2006
How to Cite
KIRSCHVINK, N., de MOFFARTS, B., FARNIR, F., PINCEMAIL, J. and LEKEUX, P. (2006), Investigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds. Equine Veterinary Journal, 38: 239–244. doi: 10.1111/j.2042-3306.2006.tb05546.x
- Issue published online: 10 JUN 2010
- Article first published online: 10 JUN 2010
- blood oxidant/antioxidant markers;
- field data;
Reasons for performing study: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently recommended. However, it is unknown whether there is a specific need for antioxidants according to performance, breed, gender or age.
Objectives: To assess whether breed-, gender- and age-related differences of blood oxidant/antioxidant markers occur in competition horses.
Methods: Healthy horses (n = 493) underwent oxidant/antioxidant blood marker determination. Vitamin E, lipophilic antioxidant capacity (ACL), ascorbic acid (AA), glutathione (GSH, GSSG), gluthione peroxidase (GPx), superoxide dismutase (SOD), selenium (Se), copper (Cu), zinc (Zn), lipid peroxides (Pool), oxidised proteins (Protox) were determined, as well as magnesium (Mg), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), packed cell volume (PCV) and haemoglobin (Hb). A mixed linear model assessed the effect of breed, gender and age category. P<0.05 was considered significant.
Results: Thoroughbreds showed the highest values of vitamin E, ACL, GPx, PCV and Hb, whilst standardbreds had the highest values of AA and LDH. Jumping horses had the highest Protox values. Females had significantly higher SOD values, whereas most of the other markers were higher in stallions and geldings. Horses age 2–6 years had higher AA, SOD and LDH values than horses age >6 years. Correlation analyses were positive and significant between vitamin E and GPx, VitE and ACL, Se and GPx, Cu and Pool and negative between Pool and vitamin E, Pool and ACL, Protox and GPx, Protox and vitamin E.
Conclusions: Blood oxidant/ antioxidant status of horses is influenced by breed, gender and age. The correlation analyses suggest synergistic relations between GPx, vitamin E and Se and an antagonistic relation between Protox-GPx, Protox-vitamin E, and Pool-vitamin E.
Potential relevance: The results of this investigation provide definition of the specific need for antioxidants and vitamins in competition horses.