Reasons for performing study: Many nutraceuticals are used as equine supplements without their efficacy having been scientifically tested. Black tea, cranberries, orange peel and ginger are a few of those nutraceuticals that warrant further study.
Objective: To test the effects of single doses of black tea, cranberry, orange peel and ginger extract on markers of oxidative stress and antioxidant status following exercise in horses.
Methods: In Study 1, 9 mature, healthy but unfit Standardbred mares were administered 2 l of a control (water), orange peel extract (30 g extract) or decaffeinated black tea extract (28 g extract). In Study 2 the same mares were administered 2 l of a control (water), cranberry extract (30 g extract) or ginger extract (30 g extract). In each study, mares were given the extracts via nasogastric tube 1 h before performing a graded exercise test (GXT), in a randomised crossover design with at least 7 days between GXTs. Blood samples were collected at rest, at fatigue, and 1 and 24 h post exercise and analysed for lipid hydroperoxides (LPO), total glutathione (GSH-T), glutathione peroxidase (GPx), α-tocopherol (TOC), β-carotene (BC) and retinol. Data were statistically analysed using a repeated measures ANOVA.
Results: In Study 1 there was no effect of treatment for LPO, GSH-T, GPx, TOC or BC. Retinol was higher for both tea (P = 0.0006) and water (P = 0.004) than for orange peel. In Study 2 there was no treatment effect for LPO, GPx, GSH-T, RET, BC or TOC.
Conclusions: The results show that a single dose of various nutraceuticals in exercising horses do not produce an effect on either oxidative stress or antioxidant status and further investigation is needed as to whether long-term supplementation would enhance these effects.