Assessment of glucose disposal with the hyperglycaemic clamp technique during low intensity exercise in Warmblood horses
Article first published online: 8 NOV 2010
© 2010 EVJ Ltd
Equine Veterinary Journal
Special Issue: Proceedings of the 8th International Conference on Equine Exercise Physiology
Volume 42, Issue Supplement s38, pages 147–151, November 2010
How to Cite
KEMMINK, A., WESTERMANN, C. M. and Van Der KOLK, J. H. (2010), Assessment of glucose disposal with the hyperglycaemic clamp technique during low intensity exercise in Warmblood horses. Equine Veterinary Journal, 42: 147–151. doi: 10.1111/j.2042-3306.2010.00284.x
- Issue published online: 8 NOV 2010
- Article first published online: 8 NOV 2010
- [Paper received for publication 07.01.10; Accepted 30.06.10]
- low intensity exercise;
- glucose disposal;
- hyperglycaemic clamp
Reasons for performing study: The quantity of glucose disposal during exercise (walk and trot) compared to rest by use of the hyperglycaemic clamp technique has not been reported previously and has relevance to nutritional requirements.
Hypothesis: Exercise (walk and trot) significantly increases glucose disposal compared to rest.
Methods: Seven healthy Dutch Warmblood mares, all in dioestrus, mean ± s.d. age 11.6 ± 2.4 years and weighing 569 ± 40 kg were fasted for 12 h prior to a hyperglycaemic clamp at rest (maintaining a steady state of the blood glucose concentration during 30 min), walk (10 min, 1.5 m/s), trot (20 min, 4.4 m/s), walk (10 min, 1.5 m/s) and rest again (maintaining a steady state during 30 min). Plasma glucose concentrations were measured every 5 min. The mean rate of glucose disposal was calculated by corrections for glucose loss via the glucose space and urine. A one-way ANOVA with post hoc Bonferroni was performed.
Results: The mean ± s.d. rate of glucose disposal was 15.0 ± 2.1 at first rest, 25.1 ± 6.2 at first walk, 37.4 ± 9.1 at trot, 33.0 ± 13.1 at second walk and 18.7 ± 4.6 µmol/kg bwt/min at second rest. Values at trot and at second walk differed significantly from values at first rest, whereas values at both rests were similar as well as at first rest and at first walk.
Conclusions: Mean rate of glucose disposal of Warmblood horses increased 2.5 times during trot compared to basal.
Potential relevance: The hyperglycaemic clamp technique is an attractive nonisotope method to assess the rate of glucose disposal in exercising horses.