Fig S1: Schematic representation of lamellar biopsy sites. (a) The right and left fore hoof depicting the alternating temporal pattern of biopsy sites. (b) Location of the 48 h samples obtained from both the central and lateral areas of the hoof below earlier biopsy sites. L = lateral, C = central, M = medial.

Fig S2: Localisation of Ln-332 subunits during the acute phase of laminitis. In lamellar tissue from normal horses, strong reactivity is observed along the perimeter of the SELs for Ln-332 as well as the α3 and β3 subunits. Loss of reactivity for each subunit was observed along the SELs in the acute phase of laminitis induction (arrows). Representative images from immunofluorescence analysis are shown. PEL = primary epidermal lamellae. SEL = secondary epidermal lamellae. Scale bar = 30 μm.

Fig S3: Schematic representation of rat Ln-332 and the γ2 processing sites for MT1-MMP and MMP-2. The full length Ln-332 isoform, made up of the α3, β3 and γ2subunits in a cruciform arrangement is shown on the left side of the figure. Cleavage of the γ2 subunit by either MMP-2 or MT1-MMP can result in the products shown on the right side of the figure. Processing of the full length γ2 subunit can result in formation of both the γ2' (∼105 kDa) and γ2x (∼80 kDa) forms which remain attached to the heterotrimer. Additionally, smaller domain fragments approximately 30–70 kDa can also be freely released from the N-terminal arm (LEb, L4-LEa, LEb-LEa).

EVJ_292_sm_fig_o1.tif2832KSupporting info item
EVJ_292_sm_Figure_o2.tif2718KSupporting info item
EVJ_292_sm_fig_o3.tif5507KSupporting info item

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