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Keywords:

  • horse;
  • adipose tissue;
  • mesenteric;
  • subcutaneous;
  • cell culture;
  • preadipocyte

Summary

Culturing adipocytes enables fine control of experimental conditions and helps minimise animal use. This report describes an explant-based method for isolating stromal-vascular cells from equine adipose tissue that enables use of small amounts of tissue. Subcutaneous and mesenteric adipose tissues were harvested post mortem and stromal-vascular cells grown from explants, prior to testing the capacity of several differentiation media to induce lipid droplet formation and increase transcript abundance of adipocyte markers. Inclusion of rosiglitazone at 1 and 5 µmol/l concentrations, along with other media components, induced differentiation of cultured equine stromal-vascular cells derived from subcutaneous and mesenteric adipose tissues.