Kojic Acid, a Cosmetic Skin Whitening Agent, is a Slow-binding Inhibitor of Catecholase Activity of Tyrosinase

Authors

  • JUANA CABANES,

    1. Departamento de Bioquimíca y Biología Molecular A, Unidad docente de Biologia, Facultad de Veterinaria, Universidad de Murcia, 30071 Espinardo, Murcia, Spain
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  • SOLEDAD CHAZARRA,

    1. Departamento de Bioquimíca y Biología Molecular A, Unidad docente de Biologia, Facultad de Veterinaria, Universidad de Murcia, 30071 Espinardo, Murcia, Spain
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  • FRANCISCO GARCIA-CARMONA

    Corresponding author
    1. Departamento de Bioquimíca y Biología Molecular A, Unidad docente de Biologia, Facultad de Veterinaria, Universidad de Murcia, 30071 Espinardo, Murcia, Spain
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Departamento de Bioquímica y Biología Molecular A, Unidad docente e Biologia, Facultad de Veterinaria, Universidad de Murcia, 30071 Espinardo, Murcia, Spain.

Abstract

Abstract— It was found that kojic acid, which is used in cosmetics for its excellent whitening effect, inhibits catecholase activity of tyrosinase in a non-classical manner. A decrease in the initial velocity to a steady-state inhibited velocity can be observed over a few minutes. This time-dependence, which is unaltered by prior incubation of the enzyme with the inhibitor, is consistent with a first-order transition. The kinetic data obtained correspond to those for a postulated mechanism that involves the rapid formation of an enzyme inhibitor complex that subsequently undergoes a relatively slow reversible reaction. Kinetic parameters characterizing this type of inhibition were evaluated by means of nonlinear regression of product accumulation curves.

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