Co-nanoencapsulated doxorubicin and Dz13 control osteosarcoma progression in a murine model
Article first published online: 2 AUG 2012
© 2012 The Authors. JPP © 2012 Royal Pharmaceutical Society
Journal of Pharmacy and Pharmacology
Volume 65, Issue 1, pages 35–43, January 2013
How to Cite
Tan, M. L., Friedhuber, A. M. and Dass, C. R. (2013), Co-nanoencapsulated doxorubicin and Dz13 control osteosarcoma progression in a murine model. Journal of Pharmacy and Pharmacology, 65: 35–43. doi: 10.1111/j.2042-7158.2012.01572.x
- Issue published online: 5 DEC 2012
- Article first published online: 2 AUG 2012
- Received April 17, 2012; Accepted June 29, 2012
- drug delivery;
Objectives Chitosan is a green (natural, abundant, biodegradable, biocompatible) biopolymer that can be formulated to encapsulate a variety of therapeutic compounds. This study aimed to investigate chitosan nanoparticles (NPs) as a means of improving delivery of the clinically used anti-cancer agent doxorubicin (Dox) and the preclinical lead compound Dz13 oligonucleotide together.
Methods A novel chitosan NP system encapsulating Dox and Dz13 was designed, biophysically characterised and tested in a clinically relevant model of the metastasising bone tumour, osteosarcoma (OS).
Key findings By careful alteration of the concentration of the individual components, a final formulation of Dz13-Dox NPs (DDNPs) was achieved, with high (>91%) loading of both compounds, which consisted of individual 50-nm particles forming aggregates as large as 500 nm, with a large positive ζ-potential. The DDNPs could be stored at various temperatures for a week without loss in activity but were prone to degradation in serum. DDNPs successfully inhibited OS tumour growth more effectively than treatment with NPs of Dz13 and Dox-chitosan, as well as Dox administered intraperitoneally. Apart from inhibiting tumour growth, DDNPs protected the affected bone from substantial destruction by aggressive tumour growth and reduced the incidence of metastasis to the lungs without causing adverse effects in mice.
Conclusion This NP is a promising formulation that could be useful for clinical management of OS.