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andr1-sup-0001.tifimage/tif77KFigure S1. Breeding strategy to generate Hsp2a-Cre; Meig1flox/flox mice. Meig1flox/flox males were crossed with Hsp2a-Cre females, the resulting females carrying Cre allele were backcrossed with the Meig1flox/flox males to generate Hsp2a-Cre; Meig1flox/flox mice. Meig1flox/flox males were used as controls.
andr1-sup-0002.tifimage/tif168KFigure S2. Identification of the Hsp2a-Cre; Meig1flox/flox mice. (A) Mice genotyping by PCR. DNA isolated from tail slips was used for PCR with a primer set flanking the left loxP site (X) (upper panel) and a primer set to amplify the Cre recombinase gene (lower panel). The lower band in the upper panel represents the wild-type allele; the upper band is the floxed allele. (B) PCR using the primer set flanking the floxed region. The upper band represents the intact allele; the lower band represents the deletion allele. The deletion bands were only present in the total testis and germ cells. Cmv-Cre; Meig1flox/flox mouse is a control.
andr1-sup-0003.tifimage/tif75KFigure S3. Breeding strategy to generate Prm-Cre; Meig1flox/flox mice. Meig1flox/flox males were crossed with Prm-Cre females, the resulting females carrying Cre allele were backcrossed with the Meig1flox/flox males to generate Prm-Cre; Meig1flox/flox mice. Meig1flox/flox males were used as controls.
andr1-sup-0004.tifimage/tif139KFigure S4. Identification of the Prm-Cre; Meig1flox/flox mice. (A) Mice genotyping by PCR. DNA isolated from tail slips was used for PCR with a primer set flanking the left loxP site (X) (upper panel), and a primer set to amplify the Cre recombinase gene (lower panel). The lower band in the upper panel represents the wild-type allele; the upper band is the floxed allele. (B) PCR using the primer set flanking the floxed region. The upper band represents the intact allele; the lower band represents the deletion allele. Notice that the deletion band is only present in germ cells. Cmv-Cre; Meig1flox/flox mouse is a control.
andr1-sup-0005.tifimage/tif76KFigure S5. Breeding strategy to generate Amh-Cre; Meig1flox/flox mice. Meig1flox/flox males were crossed with Amh-Cre females, the resulting females carrying Cre allele were backcrossed with the Meig1flox/flox males to generate Amh-Cre; Meig1flox/flox mice. Meig1flox/flox males were used as controls.
andr1-sup-0006.tifimage/tif161KFigure S6. Identification of the Amh-Cre; Meig1flox/flox mice. (A) Genotyping by PCR. DNA isolated from tail slips was used for PCR with a primer set flanking the left loxP site (X) (upper panel), and a primer set to amplify the Cre recombinase gene (lower panel). The lower band in the upper panel represents the wild-type allele; the upper band is the floxed allele. (B) PCR using the primer set flanking the floxed region. The upper band represents the intact allele; the lower band represents the allele with the deletion. Notice that the deletion band is only present in the total testis and somatic cells. Cmv-Cre; Meig1flox/flox mouse is a control.
andr1-sup-0007-SupplementalFigurelegend.docWord document23K 

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