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andr70-sup-0001-FigS1.tifimage/tif304KFigure S1. Western blot analysis of 10 week of C3HeB/FeJ wild type testes (40 μg, 10 weeks of age) using N-terminal and C-terminal GOLGA3 antibodies (1 : 1500) pre-absorbed (left) with peptide 1 : 1 and unabsorbed (right). Faint bands are evident in the pre-absorbed lanes, suggesting that the peptide concentration was not high enough to absorb all the antibody or the antibodies recognize a nonspecific protein product at the same molecular weight. In addition, western analysis shown in Fig. 3 does not support the latter hypothesis.
andr70-sup-0002-FigS2.tifimage/tif1050KFigure S2. Testes/body weight ratios. Average paired testes weights normalized to body weight (TW/BW) in mixed C3Fe;B6-Golga3repro27 mutant mice compared with heterozygous and wild type controls (±SEM). Mutant TW/BW ratios were significantly lower by 21 days post-partum (*< 0.05).
andr70-sup-0003-FigS3.tifimage/tif1050KFigure S3. Germ cell quantification. The average total number of germ cells per tubule cross section (±SEM) in mixed C3Fe;B6-Golga3repro27 mutant mice (m/m) compared with heterozygous (m/+) and wild type (+/+) controls. Twenty tubule cross sections were counted from at least three mice per genotype. The germ cell numbers differ from 12–15 dpp, but were significantly lower in mutant testes compared with controls by 18 days post-partum (*p < 0.05).
andr70-sup-0004-FigS4.tifimage/tif851KFigure S4. Percentage of post-meiotic germ cells (round and elongating) per total number of germ cells per tubule in mixed C3Fe;B6-Golga3repro27 mutant (m/m) mice compared with heterozygous (m/+) controls (±SEM). Twenty tubule cross sections were counted from at least three mice per genotype.
andr70-sup-0005-FigS5.tifimage/tif423KFigure S5. Seminal vesicle weights in mixed C3Fe;B6-Golga3repro27 mutant mice (m/m) compared with heterozygous (m/+) and wild type (+/+) controls from 2 to 20 weeks of age. Data represent mean (±SEM) in at least two mice per genotype (most time points represented by at least four mice. While the seminal vesicle weight was significantly different in mutant mice at 10 weeks of age (n = 6 mice for m/m and m/+, n = 3 for +/+), no other time points were statistically different (*p < 0.05).

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