Establishment and Epitope Analysis of Allo-Specific Cytotoxic T Lymphocytes at a Tumor Site Recognizing a Spouse's HLA-A0206 Molecule

Authors

  • NAOKO SEKI,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • AKIRA YAMADA,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • YUICHI SUEFUJI,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • TAKASHI MINE,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • SHOKO TANAKA,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • SHIN-YA GOMI,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • NOBUTOSHI KAWAGOE,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • KIKUO KOUFUJI,

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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  • KYOGO ITOH

    1. Department of Immunology, Kurume University School of Medicine, and Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Kurume 830-0011, Japan
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Address reprint requests to Akira Yamada, Ph.D., Cancer Vaccine Development Division, Kurume University Research Center for Innovative Cancer Therapy, Asahi-machi, Kurume, Fukuoka 830-0011, Japan.

E-mail: akiymd@med.kurume-u.ac.jp

Abstract

PROBLEM: The molecular basis of allo-reactivity in reproductive immunity has not been fully clarified.
METHOD OF STUDY: Cytotoxic T lymphocytes (CTLs) were established from tumor-infiltrating lymphocytes (TILs). The allo-reactivity of the CTLs against various tumor cell lines or human leukocyte antigen (HLA)-A allele-transfected COS-7 cells was measured by Cr-release or interferon-Γ production assay.
RESULTS AND CONCLUSIONS: We have established CTLs reacting to an HLA-A0206 molecule that matched a spouse's HLA-A allele from the TILs of a 68-year-old multiparous patient with gastric cancer. The amino acids at positions 66 and 88 in the α1 domain of HLA-A0206, both of which were common in the other HLA-A2 subtypes, were involved in the recognition by the CTLs. Endogenous peptides in the groove were not involved in the recognition. These results suggest the presence of long-lasting memory CTLs raised by the reproduction process, and may facilitate a better understanding of the molecular basis of allo-recognition during reproduction.

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