Painting Qa-2 onto Ped slow Preimplantation Embryos Increases the Rate of Cleavage

Authors

  • ABIGAIL S. McELHINNY,

  • GINGER E. EXLEY,

  • CAROL M. WARNER


Address reprint requests to Carol M. Warner, Northeastern University, Biology Department, 414 Mugar, 360 Huntington Ave., Boston, MA 02115.

E-mail address: cmw@neu.edu

Abstract

PROBLEM: Qa-2 protein, the Ped gene product, is linked to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Some GPI-linked proteins can be spontaneously incorporated into the membranes of cells via a technique called “protein painting.” We investigated whether Qa-2 could be painted onto T cells and embryos and whether the painted protein would be functional.
METHOD OF STUDY: Incorporation of Qa-2 into the membranes of T cells and embryos was measured by FACScan and Immuno-PCR, respectively. Function of Qa-2 was measured by cell proliferation.
RESULTS: Qa-2 was incorporated by T cells and embryos and was functional.
CONCLUSION: GPI-linked Qa-2 protein “painted” onto both T cells and preimplantation embryos is functional, as shown by increased proliferation of T cells after cross-linking with anti-Qa-2 antibody, and increased rate of cleavage division of the embryos.

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